Electronic Theses and Dissertation

ABSTRAK

Siput Faunus ater dikenal juga dengan nama Chue yang dimanfaatkan dan dikomsumsi oleh masyarakat Aceh sebagai salah satu makanan khas berupa gulai Chue. Faunus ater termasuk ke dalam kelas Gastropoda yang menurut beberapa penelitian di habitat alaminya terinfeksi oleh Salmonella sp. Penelitian ini bertujuan mengetahui adanya bakteri Salmonella sp. dan endotoksin pada Chue yang digulai di Rumah Makan Kota Banda Aceh. Penelitian dilakukan menggunakan sebanyak 25 ekor Chue yang digulai diambil dari 5 rumah makan masing-masing 5 ekor Chue, yang kemudian dijadikan sebagai 5 sampel penelitian. Isolasi dan identifikasi bakteri pada Chue yang digulai menggunakan metode Carter yang dimodifikasi sebagai pendekatan mikrobiologi. Terhadap sampel yang sama dilakukan deteksi gen Invation-A (Inv-A) dan gen Paratosa (Prt) menggunakan metode PCR. Hasil penelitian menunjukkan adanya bakteri Salmonella typhymurium menggunakan analisis secara mikrobiologi dan secara biologi molekuler tidak terdeteksi adanya gen Invation-A (Inv-A) dan gen Paratosa (Prt) pada Chue yang digulai. Dapat disimpulkan secara mikrobiologi ditemukan adanya bakteri Salmonella typhymurium pada 2 sampel Chue yang digulai dan secara biologi molekuler tidak terdeteksi gen Inv-A Salmonella dan gen Prt endotoksin pada Chue yang digulai di Rumah Makan Kota Banda Aceh.

Kata kunci : Siput Faunus ater, Gulai Chue, Salmonella sp.

ABSTRACT Faunus ater snail is also known as Chue which is used and consumed by the people of Aceh as one of the typical foods like gulai Chue. Faunus ater is belongs to the class Gastropod which is according to several studies in natural habitat has been infected by Salmonella sp. This study aims to determine the presence of Salmonella sp. and endotoxin in Chue has been curried in Banda Aceh City Restaurant. The study was conducted using as many as 25 Chue has been curried from 5 restaurants, each restaurants were taken 5 Chue and which were then used as 5 research samples. Isolation and identification of bacteria in Chue has been curried using a modified Carter method as a microbiological approach. In the same sample, the Invation-A (Inv-A) and Paratosa (Prt) genes will detect by using the PCR method. The results showed the presence of Salmonella typhimurium bacteria using microbiological analysis and molecular biology did not detect any Invation-A (Inv-A) and Paratosa (Prt) genes in Chue has been curried. It can be concluded that microbiologically the presence of Salmonella typhimurium bacteria was found in 2 samples of Chue has been curried and molecular biology did not detect the Salmonella Inv-A gene and the endotoxin Prt gene in Chue has been curried at the Banda Aceh City Restaurant. Keywords: Faunus ater snail, Gulai Chue, Salmonella sp.