Electronic Theses and Dissertation

Infeksi parasit Trypanosoma evansi dapat menyebabkan kerusakan jaringan organ limpa melalui mekanisme stres oksidatif. Daun kecombrang (Etlingera elatior) mengandung flavonoid dan fenolik yang berpotensi sebagai antioksidan dan antitripanosoma untuk meminimalkan kerusakan seluler. Penelitian ini bertujuan untuk mengetahui gambaran histopatologi organ limpa pada tikus putih yang diinfeksikan T. evansi dan diberi ekstrak daun kecombrang. Penelitian dilakukan menggunakan rancangan acak lengkap (RAL) dengan lima kelompok perlakuan, yaitu kontrol negatif (K1), kontrol positif (K2), serta tiga kelompok dosis perlakuan yaitu, 300 mg (K3), 600 mg (K4), dan 1200 mg (K5). Parameter yang diamati meliputi jumlah sel deplesi limfoid, hemosiderin, dan nekrosis yang dianalisis menggunakan uji ANOVA dan uji lanjut Duncan. Hasil penelitian menunjukkan bahwa pemberian ekstrak daun kecombrang mampu menurunkan jumlah lesi histopatologi limpa secara signifikan (P < 0,05). Dosis 600 mg (K4) menunjukkan efektivitas paling optimal dalam memperbaiki gambaran limpa. Kesimpulannya, ekstrak daun kecombrang berpotensi memperbaiki kerusakan histopatologi limpa tikus yang diinfeksi oleh T. evansi.

Infection with Trypanosoma evansi induces splenic tissue damage primarily through oxidative stress-mediated mechanisms. Etlingera elatior (torch ginger) leaves are rich in flavonoids and phenolic compounds with antioxidant and antitrypanosomal properties, suggesting their potential to mitigate parasite-induced cellular damage. This study aimed to evaluate the histopathological changes in the spleen of white rats (Rattus norvegicus) experimentally infected with T. evansi following treatment with E. elatior leaf extract. A completely randomized design (CRD) was employed, consisting of five treatment groups: a negative control (K1), a positive control (K2), and three treatment groups receiving E. elatior leaf extract at doses of 300 mg/kg bw (K3), 600 mg/kg bw (K4), and 1200 mg/kg bw (K5). Histopathological parameters, including lymphoid depletion, hemosiderin deposition, and necrosis, were evaluated and analyzed using one-way analysis of variance (ANOVA) followed by Duncan’s multiple range test. Administration of E. elatior leaf extract significantly reduced the severity of splenic histopathological lesions compared with the untreated infected group (P < 0.05). Among the tested doses, 600 mg/kg bw (K4) demonstrated the greatest efficacy in improving splenic histological architecture. These findings suggest that E. elatior leaf extract possesses therapeutic potential in ameliorating splenic histopathological damage induced by T. evansi infection.