Electronic Theses and Dissertation

Escherichia coli merupakan salah satu golongan Enterobactericiae yang ditemukan di lingkungan dan dapat menghasilkan extended spectrum beta lactamase atau ESBL. Penelitian ini bertujuan mendeteksi dan mengisolasi E. coli penghasil ESBL dari air pencucian karkas unggas di pasar Al-Mahirah Kota Banda Aceh dan resisten terhadap antibiotik. Sampel diambil menggunakan metode purposive sampling. Berdasarkan rumus slovin, dari 88 pedagang diperoleh sampel berjumlah 47 sampel yang diambil dari air bekas pencucian karkas unggas. Data hasil penelitian dianalisis secara deskriptif. Pengujian identifikasi Escherichia coli menggunakan media selektif yaitu Eosin Methylene Blue Agar (EMBA) dan dilanjutkan pengujian resistensi antibiotik menggunakan metode disk diffusion pada media Muller Hinton agar (MHA). Hasil penelitian dari air pencucian karkas terdeteksi E. coli penghasil ESBL 10 sampel yang diperoleh prevalensi dari 47 sampel air pencucian karkas dari 88 pedagang unggas di pasar Al-Mahirah sebesar 21,3% dan dilanjutkan dengan uji resistensi antibiotik menunjukkan tingkat resistensi tertinggi terjadi pada eritromisin (100%) yang diikuti oleh sefotaksim (90%), seftazidim (70%), kanamisin (70%), ampisilin (50%), dan sulphametoxazole (40%). Hasil penelitian dapat disimpulkan bahwa air bekas pencucian karkas yang digunakan pedagang unggas di Pasar Al-Mahirah, Kota Banda Aceh terdeteksi Escherichia coli penghasil enzim Extended spectrum beta-lactamase (ESBL) yang dapat menyebabkan tingginya resistensi terhadap antibiotik.

Kata kunci: Antibiotik, Unggas, ESBL, Escherichia coli, air pencucian karkas

Escherichia coli is a group of Enterobactericiae found in the environment and can produce extended spectrum beta lactamase or ESBL. This research aims to detect and isolate ESBL-producing E. coli from poultry carcass washing water at the Al-Mahirah market in Banda Aceh City and is resistant to antibiotics. Samples were taken using the purposive sampling method. Based on the Slovin formula, 47 samples were obtained from 88 traders taken from water used to wash poultry carcasses. The research data were analyzed descriptively. Escherichia coli identification testing uses selective media, namely Eosin Methylene Blue Agar (EMBA) and is continued with antibiotic resistance testing using the disk diffusion method on Muller Hinton agar (MHA) media. The results of research from carcass washing water detected ESBL-producing E. coli in 10 samples. The prevalence obtained from 47 samples of carcass washing water from 88 poultry traders at Al-Mahirah market was 21.3% and continued with antibiotic resistance testing showing the highest level of resistance occurred in erythromycin. (100%) followed by cefotaxime (90%), ceftazidime (70%), kanamycin (70%), ampicillin (50%), and sulphametoxazole (40%). The results of the research can be concluded that the water used for washing carcasses used by poultry traders at Al-Mahirah Market, Banda Aceh City, detected Escherichia coli producing the Extended Spectrum Beta-lactamase (ESBL) enzyme which can cause high resistance to antibiotics. Key words: Antibiotics, Poultry, ESBL, Escherichia coli, water washer of carcasse