Electronic Theses and Dissertation

Sintesis monoasilgliserol (MAG) dan diasilgliserol (DAG) melalui reaksi gliserolisis Crude Palm Oil (CPO) menggunakan katalis Novozyme 435 dan Lipozyme TL IM telah berhasil dilakukan. Eksperimen dilakukan dengan sistem batch dalam reaktor yang diaduk secara mekanis pada suhu 50 C dengan jumlah katalis enzim masing-masing sebesar 5% b/b dan rasio mol CPO terhadap gliserol (1 : 2) dengan variasi waktu reaksi 1, 2, 3, 4, 5, 6, 7, 8, 16 dan 24 jam. Hasil reaksi gliserolisis CPO dianalisis menggunakan kromatografi gas. Hasil penelitian menunjukkan bahwa penggunaan Novozyme 435 pada reaksi gliserolisis CPO menghasilkan kadar MAG lebih tinggi dibandingkan menggunakan Lipozyme TL IM. Penggunaan Novozyme 435 menghasilkan MAG dan DAG masing-masing sebesar 40,08% dan 11,54% pada waktu reaksi optimum 8 jam. Penggunaan Lipozyme TL IM menghasilkan MAG dan DAG masing-masing sebesar 36,21% dan 17,38% pada waktu reaksi optimum 5 jam.

The synthesis of monoacylglycerol (MAG) and diacylglycerol (DAG) through the glycerolysis of Crude Palm Oil (CPO) using the Novozyme 435 and Lipozyme TL IM catalysts has been successfully carried out. Experiments were carried out with a batch system in a mechanically stirred reactor at temperature 50°C with the amount of enzyme catalyst is 5 % w/w and the mole ratio of CPO to glycerol (1 : 2) in reaction times of 1, 2, 3, 4, 5, 6, 7, 8, 16 and 24 hours. The products of the glycerolysis of CPO were analyzed using gas chromatography. The results showed that glycerolysis of CPO using Novozyme 435 produced higher concentration of MAG than that of Lipozyme TL IM. The utilization of Novozyme 435 produced MAG and DAG of 40.08% and 11.54% respectively at an optimum reaction time of 8 hours. The utilization of Lipozyme TL IM produced MAG and DAG of 36.21% and 17.38% respectively at an optimum reaction time of 5 hours.