Electronic Theses and Dissertation

Extended Spectrum β-Lactamase adalah enzim yang dihasilkan oleh bakteri Gram negatif seperti Escherichia coli. ESBL dapat menghidrolisis sebagian besar antimikroba sehingga dapat menyebabkan resistensi terhadap beberapa jenis antibiotik sehingga mengancam kesehatan. Penelitian ini bertujuan untuk mendeteksi adanya Escherichia coli penghasil ESBL pada tangan pedagang daging ayam di pasar Al-Mahirah kota Banda Aceh. Penelitian ini menggunakan 10 sampel tangan pedagang dengan metode observasi cross sectional dan teknik pengambilan sampel yaitu purposive sampling. Sampel swab tangan pedagang daging ayam dianalisis untuk identifikasi Escherichia coli menggunakan metode Bacteriological Analytic Manual (BAM) menggunakan Eosin Methylene Blue Agar (EMBA). Skrining bakteri Escherichia coli terhadap ESBL dilakukan dengan metode difusi cakram menggunakan antibiotik jenis β-laktam yaitu sefatoksim, ampicillin dan seftazidim diatas media Mueller Hinton Agar (MHA). Hasil pemeriksaan 10 sampel terdapat 4 pedagang yang terkontaminasi Escherichia coli penghasil ESBL (40%). Hasil penelitian dapat disimpulkan bahwa pada tangan pedagang daging ayam di Pasar Al-Mahirah Kota Banda Aceh terdapat cemaran bakteri Escherichia coli penghasil ESBL.

Kata kunci: Antibiotik, Escherichia coli, Extended Spectrum Beta Lactamase, Pasar Al-Mahirah, tangan pedagang

Extended-Spectrum β-Lactamase (ESBL) is an enzyme produced by Gram-negative bacteria such as Escherichia coli. ESBL hydrolyze a wide range of antimicrobial agents, leading to resistance to multiple antibiotics and posing a threat to public health. This study aimed to detect the presence of ESBL-producing Escherichia coli on the hands of chicken meat vendors at Al-Mahirah Market in Banda Aceh City. The study employed a purposive sampling method, involving 10 hand samples from vendors using a cross-sectional observational design. Hand swab samples from chicken meat vendors were analyzed for Escherichia coli identification using the Bacteriological Analytic Manual (BAM) method and Eosin Methylene Blue Agar (EMBA) media. Screening for ESBL-producing Escherichia coli was performed using the disc diffusion method with β-lactam antibiotics, namely cefotaxime, ampicillin, and ceftazidime on Mueller Hinton Agar (MHA) media. Among the 10 samples examined, 4 vendors were contaminated with ESBL-producing Escherichia coli (40%). The study concluded that the hands of chicken meat vendors at Al-Mahirah Market in Banda Aceh City are contaminated with ESBL-producing Escherichia coli. Keywords: Antibiotic, Escherichia coli, Extended Spectrum Beta Lactamase, Al- Mahirah Market, seller’s hand