Electronic Theses and Dissertation

Keratin adalah protein berserat yang merupakan komponen utama dari bulu, rambut, tanduk dan kuku. Keratin dapat didegradasi oleh enzim keratinase yang dihasilkan mikroorganisme seperti bakteri, archaea dan jamur. Tujuan penelitian ini adalah untuk mendapatkan bakteri termofilik penghasil keratinase termostabil dari sumber air panas Ie Seuum Kawasan Gunung Seulawah Aceh. Bakteri termofilik yang diperoleh memiliki morfologi Streptobacil, Gram negatif dan berdasarkan uji biokimia API Test CHB 50 memiliki kemiripan dengan Bacillus lentus. Bakteri keratinolitik diuji kemampuannya dalam menghasilkan keratinase pada media substrat bulu ayam. Kurva pertumbuhan bakteri dan pengujian aktivitas keratinase diukur menggunakan metode spektrofotometri dengan memvariasikan waktu inkubasi pertumbuhan bakteri (0-96 jam) pada suhu 70 oC. Kurva pertumbuhan dan produksi keratinases optimum diperoleh selama 60 jam dengan nilai OD sebesar 1,598 dan aktivitas enzim 5,29 U/mL. Karakterisasi keratinase memiliki suhu dan pH optimum 70 °C dan pH 8 dengan nilai aktivitas sebasar 12,27 U/mL. Hasil penelitian ini menunjukkan bahwa bakteri termofilik Ie Seuum Kawasan Gunung Seulawah Aceh dapat menghasilkan keratinase termostabil dan mampu mendegradasi keratin yang terdapat pada limbah bulu ayam.

Keratin is a fibrous protein that is the main component of feathers, hair, horns and nails. Keratin can be degraded by the enzyme keratinase produced by microorganisms such as bacteria, archaea and fungi. The purpose of this study was to obtain thermostable keratinase producing thermophilic bacteria from the hot springs of Ie Seuum in the Seulawah Mountain area of Aceh. The thermophilic bacteria obtained had Streptobacil morphology, Gram negative and based on the biochemical test API Test CHB 50 had similarities with Bacillus lentus. Keratinolytic bacteria were tested for their ability to produce keratinase on chicken feather substrate media. Bacterial growth curve and keratinase activity were measured using spectrophotometric methods by varying the incubation time for bacterial growth (0−96 hours) at 70 °C. The optimum growth and production curve of keratinases was obtained for 60 hours with an OD value of 1.598 and an enzyme activity of 5.29 U/mL. Characterization of keratinase has an optimum temperature and pH of 70 °C and pH 8 with an activity value of 12.27 U/mL. The results of this study indicate that the thermophilic bacteria Ie Seuum in the Seulawah Mountain Region of Aceh can produce thermostable keratinase and are able to degrade the keratin contained in chicken feather waste.