Electronic Theses and Dissertation

Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi BAP dan NAA serta interaksi kedua perlakuan terhadap pertumbuhan tunas pucuk tanaman nilam secara in vitro. Penelitian dilaksanakan di Laboratorium Kultur Jaringan Tanaman Fakultas Pertanian Universitas Syiah Kuala, Darussalam, Banda Aceh pada bulan April sampai Juli 2021. Penelitian memiliki 2 faktor, faktor pertama adalah konsentrasi BAP yang terdapat 5 taraf yaitu kontrol, 0,25 mg L-1; 0,5 mg L-1; 0,75 mg L-1 dan 1 mg L-1. Faktor kedua adalah konsentrasi NAA yang terdapar 4 taraf yaitu kontrol, 0,25 mg L-1; 0,5 mg L-1 dan 0,75 mg L-1, sehingga terdapat 20 kombinasi perlakuan dengan 6 ulangan, sehingga total 120 satuan percobaan. Peubah yang diamati adalah persentase hidup eksplan, persentase kontaminasi, umur muncul tunas, persentase pembentukan tunas, tinggi eksplan, jumlah daun, persentase muncul kalus, dan jumlah akar. Hasil penelitian menunjukkan bahwa konsentrasi BAP berpengaruh terhadap hari muncul tunas, tinggi tanaman, dan persentase muncul kalus eksplan tanaman nilam. Hari muncul tunas terbaik pada 8MST pada konsentrasi 0,5 mg L-1 BAP. Tunas eksplan tanaman nilam tertinggi sebesar 2,4cm pada konsentrasi 0,25 mg L-1 BAP. Persentase kalus terbaik sebesar 66,75% pada konsentrasi 1 mg L-1 BAP. Konsentrasi NAA berpengaruh terhadap persentase tumbuh tunas, persentase kalus dan jumlah akar. Persentase tumbuh tunas terbaik sebesar 93,4% pada konsentrasi 0,5 mg L-1 NAA. Persentase kalus terbaik yaitu 69,8% dan jumlah akar terbaik sebesar 7,8 akar pada konsentrasi 0,75 mg L-1 NAA. Terdapat kombinasi interaksi antara BAP dan NAA pada konsentrasi 0,5 mgL-1 BAP+0,5 mgL-1 NAA dan Konsentrasi 0,25 mgL-1 BAP+0,5 mgL-1 NAA
Kata kunci : BAP, in vitro, NAA, tanaman nilam

Abstract. This study aims to determine the effect of BAP and NAA concentrations and the interaction of the two treatments on the growth of patchouli shoots in vitro. The research was carried out at the Plant Tissue Culture Laboratory, Faculty of Agriculture, Syiah Kuala University, Darussalam, Banda Aceh from April to July 2021. The study had 2 factors, the first factor was the concentration of BAP which had 5 levels, namely control, 0.25 mg L-1; 0.5 mg L-1; 0.75 mg L-1and 1 mg L-1. The second factor is the concentration of NAA which has 4 levels, namely control, 0.25 mg L-1; 0.5 mg L-1and 0.75 mg L-1, so there were 20 treatment combinations with 6 replications, for a total of 120 experimental units. The variables observed were the percentage of survival of explants, percentage of contamination, age of shoot emergence, percentage of shoot formation, explant height, number of leaves, percentage of callus emergence, and number of roots. The results showed that the concentration of BAP affected the day of shoot emergence, plant height, and the percentage of callus emergence from patchouli plant explants. The day the best shoots appeared at 8MST at a concentration of 0.5 mg L-1 BAP. The highest explant shoots of patchouli were 2.4 cm at a concentration of 0.25 mg L-1 BAP. The best callus percentage was 66.75% at a concentration of 1 mg L-1 BAP. The concentration of NAA affects the percentage of shoot growth, the percentage of callus and the number of roots. The best shoot growth percentage was 93.4% at a concentration of 0.5 mg L-1 NAA. The best callus percentage was 69.8% and the best number of roots was 7.8 roots at a concentration of 0.75 mg L-1 NAA. There is a combination of interactions between BAP and NAA at a concentration of 0.5 mgL-1 BAP+0.5 mgL-1 NAA and a concentration of 0.25 mgL-1 BAP+0,5 mgL-1 NAA Keywords : BAP, in vitro, NAA, patchouli