Electronic Theses and Dissertation

ABSTRAK

Ikan lele asap merupakan salah satu bahan pangan yang dapat mengalami proses pembusukan atau terkena cemaran bakteri, salah satunya disebabkan oleh bakteri Salmonella sp. Penelitian ini bertujuan untuk mendeteksi ada tidaknya cemaran Salmonella sp. pada ikan lele asap yang ada di pasar tradisional Kecamatan Simpang Kiri Kota Subulussalam. Pengambilan 20 sampel ikan lele asap diambil secara acak dari 5 pedagang ikan lele asap di pasar tradisional Kecamatan Simpang Kiri Kota Subulussalam. Ikan lele ditimbang sebanyak 5 gr dan dimasukkan ke dalam media Lactosa Broth (LB) dan diinkubasikan pada suhu 37ºC selama 24 jam. Selanjutnya diambil 1 ml dari media Lactosa Broth (LB) dan dimasukkan ke dalam media Selenite Cystine Broth (SCB) dan diinkubasikan pada suhu 37ºC selama 24 jam. Selanjutnya dilakukan penanaman pada media Salmonella Shigella Agar (SSA), dan diinkubasikan pada suhu 37ºC selama 24 jam dengan posisi terbalik. Pada SSA dilakukan perhitungan secara menyeluruh setelah 24 jam dan koloni yang dihitung ialah koloni bakteri yang berwarna hitam di dalam cawan petri. Analisis data dilakukan secara deskriptif. Berdasarkan hasil penelitian didapatkan hasil 2 sampel terdeteksi adanya Salmonella sp. pada media SSA, dengan koloni berwarna hitam.

Kata kunci : Ikan lele asap, Kota Subulussalam, Salmonella sp.

ABSTRACT

Smoked catfish is one of the many foods that can undergo a process of decay or exposure to bacterial contamination, one of which is caused by the bacteria Salmonella sp. This study aims to detect the presence or absence of Salmonella sp. on smoked catfish in the traditional markets of Simpang Kiri District, Subulussalam City. Sampling of 20 smoked catfish was taken randomly from 5 smoked catfish sellers in the traditional markets of Simpang Kiri District, Subulussalam City. The catfish was weighed as much as 5 g and put into Lactosa Broth (LB) medium, and incubated at 37ºC for 24 hours. Furthermore, 1 ml was taken from the Lactosa Broth (LB) medium and put into the Selenite Cystine Broth (SCB) medium and incubated at 37ºC for 24 hours. Furthermore, planting was carried out on Salmonella Shigella Agar (SSA) media, and incubated at 37ºC for 24 hours in reverse position. In SSA, a comprehensive count was carried out after 24 hours, and the colonies that were counted were black bacterial colonies in a petri dish. Data analysis was carried out descriptively. Based on the research results, it was found that 2 samples were detected by Salmonella sp. in the SSA media, with black bacterial colonies.

Key words : Smoked catfish, Subulussalam city, Salmonella sp.

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