Electronic Theses and Dissertation

Kurkumin merupakan senyawa bioaktif utama dalam kunyit (Curcuma longa L.) yang memiliki berbagai manfaat farmakologis, namun pemisahan dan pemurniannya masih memerlukan metode yang efisien. Penelitian ini bertujuan mengevaluasi kinerja membran kromatografi berbasis Mixed Matrix Membrane Adsorbers (MMMAs) berbahan selulosa asetat yang dimodifikasi dengan silika abu sekam padi untuk pemisahan dan pemurnian kurkumin secara kontinu. Membran dikarakterisasi melalui uji sifat mekanik, fluks, FTIR (Fourier Transform Infrared Spectroscopy), XRD (X-Ray Diffraction), SEM (Scanning Electron Microscopy), DTA-TGA (Differential Thermal Analysis-Thermogravimetric Analysis), porositas,dan derajat pengembangan. Proses pemisahan dilakukan menggunakan sistem dead-end filtration melalui tahap loading dan elusi, kemudian dianalisis menggunakan spektrofotometri UV-Vis (Ultraviolet-Visible) dan KLT (Kromatografi Lapis Tipis). Hasil penelitian menunjukkan bahwa membran SA12%-Si40% memiliki sifat mekanik dan permeabilitas yang baik dengan kuat tarik 2,49 Kgf/mm², elongasi 15,76%, dan fluks 181,98 L/m²·jam. Penggunaan dua membran meningkatkan kapasitas adsorpsi dibandingkan membran tunggal. Kondisi elusi optimum diperoleh menggunakan pelarut DCM:metanol (50:50) dengan recovery kurkumin sebesar 89,16%, sedangkan pemisahan ekstrak kunyit menghasilkan kapasitas adsorpsi 0,292 mg/g dan recovery 90,32%. Hasil penelitian menunjukkan bahwa membran kromatografi berbasis MMMAs berpotensi sebagai metode pemisahan dan pemurnian kurkumin yang efisien dan ramah lingkungan.

Kata kunci: kurkumin, membran kromatografi, Mixed Matrix Membrane Adsorbers (MMMAs), selulosa asetat, silika abu sekam padi.

Curcumin is the main bioactive compound in turmeric (Curcuma longa L.) with various pharmacological properties; however, its separation and purification still require an efficient method. This study aimed to evaluate the performance of a chromatography membrane based on Mixed Matrix Membrane Adsorbers (MMMAs) consisting of cellulose acetate modified with rice husk ash silica for the continuous separation and purification of curcumin. The membrane was characterized by mechanical strength, flux, FTIR (Fourier Transform Infrared Spectroscopy), XRD (X-Ray Diffraction), SEM (Scanning Electron Microscopy), DTA-TGA (Differential Thermal Analysis-Thermogravimetric Analysis), porosity, and swelling degree analyses. The separation process was carried out using a dead-end filtration system through loading and elution stages, followed by UV-Vis (Ultraviolet-Visible) spectrophotometric and TLC (Thin-Layer Chromatography) analyses. The results showed that the SA12%-Si40% membrane exhibited good mechanical and permeability properties, with a tensile strength of 2.49 Kgf/mm², an elongation of 15.76%, and a flux of 181.98 L/m²·h. The use of two membranes increased the adsorption capacity compared with a single membrane. The optimum elution condition was achieved using a dichloromethane (50:50) solvent system, resulting in a curcumin recovery of 89.16%, while the separation of turmeric extract produced an adsorption capacity of 0.292 mg/g and a recovery of 90.32%. These findings demonstrate that the MMMAs-based chromatography membrane has considerable potential as an efficient and environmentally friendly method for curcumin separation and purification. Keywords: curcumin, chromatography membrane, Mixed Matrix Membrane Adsorbers (MMMAs), cellulose acetate, rice husk ash silica.