Electronic Theses and Dissertation

Rusa sambar (Rusa unicolor) merupakan satwa dengan status vulnerable (rentan) menurut IUCN yang memerlukan upaya konservasi serius, termasuk pemantauan reproduksi secara berkelanjutan. Pengukuran hormon progesteron secara non-invasif melalui sampel feses menjadi alternatif yang lebih aman karena tidak menimbulkan stres pada hewan. Namun, penggunaan kit ELISA komersial pada sampel feses rusa sambar belum pernah dilaporkan sehingga diperlukan uji validasi. Penelitian ini bertujuan untuk menguji akurasi dan presisi kit ELISA progesteron komersial (EIA DRG 1561) dalam mengukur kadar progesteron dari sampel feses rusa sambar. Sampel feses diambil dari 6 ekor rusa sambar betina dewasa di Wisata Alam Taman Rusa Lamtanjong, Kabupaten Aceh Besar, Aceh. Feses diekstraksi menggunakan metanol 80% dan diukur menggunakan kit ELISA DRG 1561. Uji akurasi dilakukan dengan menghitung persentase recovery antara konsentrasi standar yang diketahui dengan konsentrasi yang terukur, sedangkan uji presisi dievaluasi melalui nilai coefficient of variation (% CV) pada uji intra-assay dan inter-assay. Hasil uji akurasi menunjukkan rataan persentase recovery sebesar 100,57 ± 4,27%, dengan kisaran 96,78% – 107,52%, yang seluruhnya berada dalam rentang toleransi 90,9% – 112,0% sesuai panduan DRG International Inc. Hasil uji presisi menunjukkan nilai % CV intra-assay berkisar antara 1,38% – 5,12%, dan nilai % CV inter-assay berkisar antara 0,22% – 8,66%, yang seluruhnya berada di bawah ambang batas 15% sesuai kriteria FDA dan EMA. Berdasarkan hasil tersebut, disimpulkan bahwa kit ELISA komersial DRG 1561 memiliki akurasi dan presisi yang baik sehingga valid digunakan untuk mengukur kadar progesteron secara non-invasif dari sampel feses rusa sambar

Sambar deer (Rusa unicolor) is classified as Vulnerable by the IUCN, necessitating serious conservation efforts including continuous reproductive monitoring. Non-invasive measurement of progesterone through fecal samples offers a safer alternative that does not induce stress in animals. However, the use of commercial ELISA kits on sambar deer fecal samples has not yet been reported, making validation studies necessary. This study aimed to evaluate the accuracy and precision of a commercial progesterone ELISA kit (EIA DRG 1561) for measuring progesterone concentrations in sambar deer feces. Fecal samples were collected from 6 adult female sambar deer at Lamtanjong Deer Park, Aceh Besar, Aceh. Samples were extracted using 80% methanol and measured using the DRG 1561 ELISA kit. Accuracy was assessed by calculating the percentage recovery between known standard concentrations and measured concentrations, while precision was evaluated through the coefficient of variation (% CV) for both intra-assay and inter-assay tests. Accuracy results showed a mean percentage recovery of 100.57 ± 4.27%, ranging from 96.78% to 107.52%, all within the acceptable tolerance range of 90.9% – 112.0% as specified by DRG International Inc. Precision results showed intra-assay % CV values ranging from 1.38% to 5.12%, and inter-assay % CV values ranging from 0.22% to 8.66%, all below the 15% threshold as required by the FDA and EMA guidelines. These results indicate that the DRG 1561 commercial ELISA kit demonstrates good accuracy and precision, and is therefore valid for non-invasive measurement of progesterone from sambar deer fecal samples