Electronic Theses and Dissertation

Nanopartikel tembaga (Cu-NPs) telah berhasil disintesis melalui metode green synthesis yang dikombinasikan dengan metode sonikasi, menggunakan ekstrak metanol kulit batang srikaya (Annona squamosa L.) sebagai agen pereduksi dan penstabil. Kandungan senyawa bioaktif pada ekstrak ditentukan melalui analisis Total Phenolic Content (TPC) dan Total Flavonoid Content (TFC). Hasil analisis menunjukkan bahwa ekstrak metanol kulit batang srikaya memiliki nilai TPC sebesar 40,94 mg GAE/g dan TFC sebesar 0,45 mg QE/g. Senyawa fenolik dan flavonoid yang terkandung dalam ekstrak diduga berperan dalam mereduksi ion Cu²⁺ menjadi nanopartikel tembaga. Pembentukan nanopartikel dikonfirmasi melalui karakterisasi menggunakan Spektrofotometer UV-Vis, Fourier Transform Infrared (FTIR), X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM) dan Transmission Electron Microscopy (TEM). Hasil analisis mengindikasikan bahwa Cu-NPs tersebut terdiri dari CuO₂, CuO, Cu, dan Cu₂O dengan dominasi oleh tembaga oksida. Nanopartiokel Cu memiliki aktivitas antibakteri terhadap Escherichia coli dan Staphylococcus aureus, dimana masing-masing zona hambatnya sebesar 18,03 mm dan 7,07 mm pada kosentrasi 20%. Penelitian ini menunjukkan bahwa hasil green synthesis nanopartikel tembaga menggunakan ekstrak metanol kulit batang srikaya dengan metode sonikasi berpotensi digunakan sebagai agen bioreduktor dalam sintesis nanopartikel tembaga.

Copper nanoparticles (Cu-NPs) were successfully synthesized using a green synthesis method combined with sonication, employing a methanol extract of srikaya (Annona squamosa L.) stem bark as a reducing agent and stabilizer. The content of bioactive compounds in the extract was determined through analysis of Total Phenolic Content (TPC) and Total Flavonoid Content (TFC). The analysis results showed that the methanol extract of srikaya stem bark had a TPC value of 40.94 mg GAE/g and a TFC value of 0.45 mg QE/g. The phenolic and flavonoid compounds contained in the extract are believed to play a role in reducing Cu²⁺ ions to copper nanoparticles. The formation of nanoparticles was confirmed through characterization using a UV-Vis spectrophotometer, Fourier Transform Infrared (FTIR), X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM), and Transmission Electron Microscopy (TEM). The analysis results indicate that the Cu-NPs consist of CuO₂, CuO, Cu, and Cu₂O, with copper oxide being the dominant component. The Cu nanoparticles exhibit antibacterial activity against Escherichia coli and Staphylococcus aureus, with inhibition zones of 18.03 mm and 7.07 mm, respectively, at a 20% concentration. This study demonstrates that the green synthesis of copper nanoparticles using methanol extract of srikaya bark via the sonication method has the potential to be used as a bioreducing agent in the synthesis of copper nanoparticles.