Electronic Theses and Dissertation

Penelitian ini bertujuan untuk menguji validitas biologis kit ELISA komersial dalam mengukur kadar hormon progesteron secara non-invasif pada rusa sambar (Rusa unicolor) menggunakan sampel feses. Monitoring hormon reproduksi secara non-invasif penting dilakukan pada satwa liar karena metode ini lebih aman, praktis, dan tidak menyebabkan stres akibat penanganan hewan. Penelitian dilakukan terhadap enam ekor rusa sambar yang terdiri atas tiga ekor betina dan tiga ekor jantan. Sampel feses dikumpulkan dan dianalisis menggunakan kit ELISA komersial untuk mengukur konsentrasi hormon progesteron. Data hasil pengukuran dianalisis menggunakan uji Independent T-test dengan bantuan program SPSS untuk mengetahui perbedaan kadar progesteron berdasarkan jenis kelamin. Hasil penelitian menunjukkan bahwa rerata konsentrasi progesteron pada rusa betina sebesar 1,370640 ± 0,740349, sedangkan pada rusa jantan sebesar 0,580717 ± 0,330617. Hasil uji Independent T-test menunjukkan nilai signifikansi (Sig. 2-tailed) sebesar 0,167 (p>0,05), sehingga tidak terdapat perbedaan yang signifikan secara statistik antara kadar progesteron rusa jantan dan betina. Meskipun demikian, rerata konsentrasi progesteron pada rusa betina lebih tinggi dibandingkan rusa jantan, yang menunjukkan adanya pola biologis hormonal sesuai fisiologi reproduksi mamalia. Progesteron pada betina diproduksi terutama oleh corpus luteum ovarium dan berperan dalam pengaturan siklus estrus serta mempertahankan kebuntingan, sedangkan pada jantan progesteron hanya diproduksi dalam jumlah kecil sebagai prekursor hormon steroid lainnya. Berdasarkan hasil penelitian dapat disimpulkan bahwa kit ELISA komersial mampu mendeteksi hormon progesteron pada sampel feses rusa sambar dan berpotensi digunakan sebagai metode non-invasif dalam monitoring reproduksi satwa liar, khususnya rusa sambar.

Kata kunci: ELISA, hormon progesteron, non-invasif, rusa sambar, validasi biologis.

This study aims to test the biological validity of a commercial ELISA kit for non-invasively measuring progesterone levels in sambar deer (Rusa unicolor) using fecal samples. Non-invasive monitoring of reproductive hormones is important in wildlife because this method is safer, more practical, and does not cause stress due to animal handling. The study was conducted on six sambar deer, consisting of three females and three males. Fecal samples were collected and analyzed using a commercial ELISA kit to measure progesterone hormone concentrations. The measurement data were analyzed using an Independent T-test with the assistance of SPSS software to determine differences in progesterone levels based on sex. The results of the study showed that the average progesterone concentration in female deer was 1.370640 ± 0.740349, while in male deer it was 0.580717 ± 0.330617. The results of the Independent T-test showed a significance value (Sig. 2-tailed) of 0.167 (p>0.05), indicating no statistically significant difference between the progesterone levels of male and female deer. Nevertheless, the average progesterone concentration in female deer was higher than in male deer, indicating a hormonal biological pattern consistent with mammalian reproductive physiology. In females, progesterone is primarily produced by the ovarian corpus luteum and plays a role in regulating the estrus cycle and maintaining pregnancy, whereas in males, progesterone is produced only in small amounts as a precursor to other steroid hormones. Based on the research results, it can be concluded that commercial ELISA kits are capable of detecting progesterone in sambar deer fecal samples and have the potential to be used as a non-invasive method for monitoring the reproduction of wild animals, particularly sambar deer. Keywords: ELISA, progesterone, non-invasive, sambar deer, biological validation.