Electronic Theses and Dissertation

Sabut pinang (Areca catechu L.) diketahui mengandung metabolit sekunder seperti
flavonoid, tanin, saponin, alkaloid, dan steroid yang berpotensi sebagai agen
antimikroba. Penelitian ini bertujuan untuk mengetahui aktivitas antimikroba ekstrak
etanol sabut pinang tua terhadap pertumbuhan Escherichia coli, Staphylococcus
aureus, dan Candida albicans. Sabut pinang diekstraksi dengan metode maserasi
menggunakan etanol 96%. Aktivitas antimikroba diuji dengan metode difusi cakram
pada konsentrasi ekstrak 5%, 15%, 30%, dan 45%. Hasil menunjukkan bahwa
ekstrak tidak memberikan efek hambat terhadap E. coli pada semua konsentrasi.
Terhadap S. aureus, hanya konsentrasi 45% yang menghasilkan zona hambat lemah
(7,49 ± 0,64 mm). Sebaliknya, ekstrak menunjukkan aktivitas antijamur kuat terhadap
C. albicans, dengan zona hambat berkisar antara 13,84 ± 12,56 mm (konsentrasi
30%) hingga 20,18 ± 17,50 mm (konsentrasi 45%). Ini mengindikasikan bahwa
ekstrak etanol sabut pinang tua memiliki potensi sebagai agen antijamur, khususnya
terhadap Candida albicans.
Kata kunci: Aktivitas antimikroba, Candida albicans, ekstrak etanol, Escherichia
coli, sabut pinang, Staphylococcus aureus.

Areca nut (Areca catechu L.) is known to contain secondary metabolites such as flavonoids, tannins, saponins, alkaloids, and steroids, which are potential antimicrobial agents. This study aims to determine the antimicrobial activity of ethanol extract of mature areca nut husk against the growth of Escherichia coli, Staphylococcus aureus, and Candida albicans. The areca nut husk was extracted using the maceration method with 96% ethanol. The antimicrobial activity was tested using the disc diffusion method with extract concentrations of 5%, 15%, 30%, and 45%. Results showed that the extract exhibited no inhibitory effect against E. coli at all concentrations. Against S. aureus, only the 45% concentration produced a weak inhibition zone (7.49 ± 0.64 mm). However, the extract demonstrated strong antifungal activity against C. albicans, with inhibition zones ranging from 13,84 ± 12,56 mm (concentration 30%) to 20,18 ± 17,50 mm (concentration 45%). These findings suggest that the ethanol extract of mature areca nut husk has potential as an antifungal agent, especially against Candida albicans. Keywords: Antimicrobial activity, areca nut husk, Candida albicans, Escherichia coli, ethanol extract, Staphylococcus aureus.