Electronic Theses and Dissertation

Latar Belakang: Staphylococcus aureus merupakan bakteri Gram-positif yang dapat berkolonisasi pada vestibulum nasi dan berpotensi menyebabkan infeksi serius, terutama pada pasien dengan faktor risiko tertentu. Keberadaan gen mecA sebagai penanda resistensi metisilin (MRSA) dan gen Panton-Valentine Leukocidin (PVL) sebagai faktor virulensi penting berperan dalam peningkatan morbiditas baik di rumah sakit maupun komunitas. Data Rumah Sakit Umum Daerah dr. Zainoel Abidin (RSUDZA) menunjukkan tingginya proporsi MRSA dari sampel klinis, sehingga diperlukan analisis perbandingan pola genetik antara fasilitas tersier dan primer.
Tujuan: Menganalisis perbandingan persentase gen mecA dan PVL pada isolat S. aureus dari Rumah Sakit Tersier dan Fasilitas Kesehatan Layanan Primer serta menggambarkan karakteristik pasien terkolonisasi.
Metode: Penelitian ini merupakan studi analitik observasional dengan desain komparatif potong lintang berbasis laboratorium dan data klinis. Sampel diperoleh melalui consecutive sampling pada pasien dengan kolonisasi S. aureus. Pemeriksaan gen mecA dan PVL dilakukan secara molekuler dengan tehnik PCR, dan analisis perbandingan menggunakan uji Chi-square.
Hasil: Tidak terdapat perbedaan bermakna persentase gen mecA antara subjek di Rumah Sakit Tersier dan Fasilitas Kesehatan Layanan Primer (p>0,05). Perbedaan yang signifikan secara statistik diamati pada persentase gen PVL antara kedua fasilitas tersebut (p

Background: Staphylococcus aureus is a Gram-positive bacterium that can colonize the nasal vestibule and has the potential to cause serious infections, particularly in patients with certain risk factors. The presence of the mecA gene, a marker of methicillin resistance (MRSA), and the Panton-Valentine Leukocidin (PVL) gene, an important virulence factor, contributes to increased morbidity in both hospital and community settings. Data from the Dr. Zainoel Abidin Regional General Hospital (RSUDZA) indicate a high proportion of MRSA in clinical samples, necessitating a comparative analysis of genetic patterns between tertiary and primary care facilities. Objective: To analyze the comparison of mecA and PVL gene percentage in S. aureus isolates from tertiary hospitals and primary care facilities, and to describe the characteristics of colonized patients. Method: This study is an observational analytical study with a comparative cross-sectional design based on laboratory and clinical data. The sample was obtained through consecutive sampling of patients with S. aureus colonization. Molecular testing for the mecA and PVL genes was performed using PCR, and comparative analysis was conducted using the chi-square test. Results: There was no statistically significant difference in the percentage of mecA genes between subjects at tertiary hospitals and primary care facilities (p > 0.05). A statistically significant difference was observed in the percentage of PVL genes between the two types of facilities (p < 0.05), indicating a variation in the percentage between tertiary and primary care facilities. Conclusion: The percentage of the PVL gene differed significantly between tertiary hospitals and primary health care facilities, whereas the mecA gene showed no significant difference. These findings are important for strategies to prevent and control MRSA infections in hospitals and the community. Keywords: Staphylococcus aureus, MRSA, mecA gene, PVL gene.