Electronic Theses and Dissertation

Jejunum merupakan salah satu organ pada saluran cerna yang berfungsi sebagai tempat penyerapan nutrisi utama pada usus halus yang diperankan oleh vili dan kripta jejunum. Penelitian ini bertujuan mengetahui histomorfometri perkembangan jejunum ayam broiler (Gallus gallus domesticus) berdasarkan umur. Penelitian ini menggunakan 25 ekor ayam broiler jantan galur MB90 yang dipelihara selama 35 hari. Sampel jejunum diambil pada umur 7, 14, 21, 28, dan 35 hari masing-masing sebanyak 3 ekor per waktu pengamatan. Sampel diproses menjadi preparat histologi dan diwarnai dengan pewarnaan hematoksilin-eosin (HE) dan alcian blue (AB). Parameter yang diamati meliputi luas permukaan vili, kedalaman kripta, dan jumlah sel goblet. Data dianalisis menggunakan uji One Way ANOVA dan dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan bahwa luas permukaan vili dan kedalaman kripta mengalami peningkatan signifikan (p0,05) namun mengalami peningkatan secara numerik. Jumlah sel goblet meningkat secara signifikan (p

The jejunum is a segment of the digestive tract that serves as the primary site of nutrient absorption in the small intestine, a function mediated by the intestinal villi and crypts. This study aimed to evaluate the histomorphometric development of the jejunum in broiler chickens (Gallus gallus domesticus) at different ages. A total of 25 male MB90 broiler chickens were reared for 35 days. Jejunal samples were collected at 7, 14, 21, 28, and 35 days of age, with three birds sampled at each observation time. The samples were processed into histological sections and stained using hematoxylin-eosin (HE) and Alcian blue (AB). The observed parameters included villus surface area, crypt depth, and goblet cell number. The data were analyzed using one-way analysis of variance (ANOVA), followed by Duncan's multiple range test. The results showed that villus surface area and crypt depth increased significantly (p < 0.05) with advancing age, particularly during the grower and finisher phases. The villus height to crypt depth ratio did not differ significantly (p > 0.05) among age groups, although it showed a numerical increase. The number of goblet cells also increased significantly (p < 0.05) with age, reaching the highest value at 35 days of age. In conclusion, the jejunal structure of broiler chickens undergoes histomorphometric development with increasing age, as indicated by increases in villus surface area, crypt depth, and goblet cell number. Keywords: Jejunum, histomorphometric development, villus surface area, crypt depth, goblet cells