Electronic Theses and Dissertation

DETEKSI RESISTENSI ANTIBIOTIK PADA BAKTERI ASAM LAKTAT DARI USUS BURUNG PUYUH (Coturnix japonica)

ABSTRAK
Bakteri Asam Laktat (BAL) diketahui berperan penting dalam menjaga keseimbangan mikrobiota usus, namun dapat menjadi reservoir gen resistensi antibiotik. Penelitian ini bertujuan untuk mendeteksi resistensi antibiotik BAL yang diisolasi dari usus burung puyuh (Coturnix japonica) yang berasal dari Peternakan Puyuh Hanin Grup. Penelitian dilakukan dengan menggunakan metode kultur, identifikasi morfologi, pewarnaan Gram, uji katalase, dan uji resistensi antibiotik menggunakan metode difusi cakram terhadap lima jenis antibiotik: gentamisin, tetrasiklin, eritromisin, siprofloksasin, dan kloramfenikol.Hasil menunjukkan bahwa isolat BAL yang diperoleh merupakan Gram positif, berbentuk batang, dan katalase negatif. Hasil uji resistensi antibiotik 10 isolat asal burung puyuh menunjukkan, sebanyak 80% isolat menunjukkan resistensi terhadap eritromisin, gentamisin, dan siprofloksasin, sementara kloramfenikol menunjukkan efektivitas paling tinggi. Temuan ini mengindikasikan adanya potensi penyebaran gen resistensi dari BAL ke bakteri patogen. Hasil uji resistensi terhadap kelima antibiotik tersebut, sebagian besar isolat menunjukkan resistensi terhadap eritromisin, gentamisin, dan siprofloksasin, sedangkan kloramfenikol menunjukkan efektivitas paling tinggi dengan seluruh isolat tergolong sensitif. Berdasarkan hasil penelitian dapat disimpulkan bahwa bakteri asam laktat dari usus burung puyuh di Peternakan Puyuh Hanin Grup, telah mengalami resistensi terhadap beberapa golongan antibiotik.

Kata kunci: bakteri asam laktat, burung puyuh, probiotik, resistensi antibiotik, zona hambat.

DETECTION OF ANTIBIOTIC RESISTANCE IN LACTIC ACID BACTERIA FROM QUAIL INTESTINAL Dikoreksi sesuai abstrak Bahasa Indonesia ABSTRACT Lactic Acid Bacteria (LAB) are known to play an important role in maintaining gut microbiota balance; however, they may also act as reservoirs of antibiotic resistance genes. This study aimed to detect antibiotic resistance in LAB isolated from the intestines of quails (Coturnix japonica) obtained from Hanin Group Quail Farm. The study was conducted using culture methods, morphological identification, Gram staining, catalase testing, and antibiotic resistance testing using the disk diffusion method against five antibiotics: gentamicin, tetracycline, erythromycin, ciprofloxacin, and chloramphenicol. The results showed that the LAB isolates were Gram-positive, rod-shaped, and catalase-negative. Antibiotic resistance testing of ten LAB isolates from quail intestines revealed that 80% of the isolates were resistant to erythromycin, gentamicin, and ciprofloxacin, while chloramphenicol exhibited the highest effectiveness. These findings indicate a potential risk of resistance gene dissemination from LAB to pathogenic bacteria. Overall, most isolates showed resistance to erythromycin, gentamicin, and ciprofloxacin, whereas chloramphenicol demonstrated the highest efficacy, with all isolates classified as sensitive. Based on the results of this study, it can be concluded that lactic acid bacteria isolated from the intestines of quails at Hanin Group Quail Farm have developed resistance to several classes of antibiotics. Keywords: antibiotic resistance, inhibition zone, lactic acid bacteria, probiotics, quail.