Electronic Theses and Dissertation

Burung kuntul besar (Egretta alba) merupakan burung air yang hidup di ekosistem
lahan basah dan berpotensi menjadi reservoir bakteri enterik, salah satunya Enterobacter
sp. Bakteri ini bersifat oportunistik dan dapat menimbulkan resiko kesehatan bagi hewan,
manusia, serta lingkungan. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi
bakteri Enterobacter sp. pada burung kuntul besar (Egretta alba) yang hidup di ekosistem
hutan mangrove Kecamatan Baitussalam, Aceh Besar. Penelitian ini dilakukan secara
observasi lapangan dan eksplorasi laboratorium menggunakan metode Carter. Sebanyak 10
sampel swab kloaka burung kuntul besar diinokulasikan ke dalam media Nutrient Broth
(NB), kemudian diisolasi menggunakan media MacConkey Agar (MCA). Koloni yang
tumbuh selanjutnya diamati morfologinya, dilakukan pewarnaan Gram, serta diuji secara
biokimia meliputi uji IMViC, TSIA, SIM, dan fermentasi gula-gula. Hasil penelitian
menunjukkan bahwa seluruh sampel mengalami pertumbuhan pada media NB, dan
menunjukkan lima sampel memperlihatkan koloni berwarna merah muda pada media
MCA. Secara pewarnaan Gram menunjukkan bakteri yang terisolasi merupakan Gram
negatif dan berbentuk basil. Uji biokimia menunjukkan hasil indol negatif, motil, MR
positif, VP negatif, SCA positif, serta mampu memfermentasi berbagai gula. Berdasarkan
hasil identifikasi, bakteri yang ditemukan merupakan Enterobacter cloacae.
Kata kunci: Enterobacter sp., kuntul besar, hutan mangrove, isolasi dan identifikasi

The great egret (Egretta alba) is a waterbird that inhabits wetland ecosystems and has potential to act as a reservoir for enteric bacteria, including Enterobacter sp. This bacterium is opportunistic in nature and can pose a health risk to animals, humans, and the environment. This study aimed to isolated and identify Enterobacter sp. from great egrets (Egretta alba) inhabiting the mangrove forest ecosystem of Baitussalam District, Aceh Besar. The study was conducted through field observation and laboratory exploration using the Carter method. A total of ten cloacal swab samples from great egrets were inoculated into Nutrient Broth (NB) and subsequently isolated on MacConkey Agar (MCA). The resulting colonies were observed for their morphological characteristics, subjected to Gram staining, and analyzed using biochemical tests including IMViC, TSIA, SIM, and carbohydrate fermentation test. The results showed that all samples exhibited growth in NB, and five samples produce pink colonies on MCA. Gram staining revealed that the isolated bacteria were Gram-negative and rod-shape. Biochemical test results indicated negative indole, motility positive, MR positive, VP negative, SCA positive, and the ability to ferment various carbohydrates. Based on the identification results, the bacterium isolated was Enterobacter cloacae. Keywords: Enterobacter sp., great egret, mangrove forest, isolation and identification