Electronic Theses and Dissertation

Tumbuhan sarang semut (Myrmecodia tuberosa) merupakan tanaman obat yang mengandung senyawa metabolit sekunder seperti flavonoid, tanin, dan saponin yang berpotensi sebagai antibakteri. Meningkatnya kasus resistensi antibiotik, khususnya pada bakteri enterik seperti Shigella sp., mendorong perlunya pencarian sumber antibakteri alternatif berbasis bahan alam. Penelitian ini bertujuan mengetahui aktivitas antibakteri ekstrak metanol umbi tumbuhan sarang semut asal Aceh terhadap pertumbuhan bakteri Shigella sp. isolat klinis. Penelitian ini merupakan studi eksperimental menggunakan rancangan acak lengkap. Perlakuan terdiri atas ekstrak metanol umbi sarang semut dengan konsentrasi 25%, 50%, dan 75%, kontrol positif ciprofloxacin, serta kontrol negatif CMC 1%, dengan tiga kali pengulangan. Parameter yang diamati adalah diameter zona hambat pertumbuhan bakteri. Hasil penelitian menunjukkan bahwa ekstrak metanol umbi sarang semut pada konsentrasi 25%, 50%, 75%, kontrol positif ciprofloxacin dan kontrol negatif CMC 1% menghasilkan diameter zona hambat berturut-turut sebesar 6,35 ± 0,67 mm, 8,53 ± 0,79 mm, 11,23 ± 1,09 mm, 26.53 ± 0.92 dan 0.00 ± 0.00. Analisis One Way ANOVA menunjukkan adanya perbedaan yang signifikan antar perlakuan (P

Ant plant (Myrmecodia tuberosa) is a medicinal plant containing secondary metabolites such as flavonoids, tannins, and saponins, which have potential antibacterial activity. The increasing incidence of antibiotic resistance, particularly in enteric bacteria such as Shigella sp., has driven the need to explore alternative antibacterial sources derived from natural products. This study aimed to evaluate the antibacterial activity of methanolic extract of ant plant tubers originating from Aceh against the growth of clinical isolates of Shigella sp. This research was an experimental study using a completely randomized design. Treatments consisted of methanolic extract of ant plant tubers at concentrations of 25%, 50%, and 75%, ciprofloxacin as a positive control, and 1% CMC as a negative control, with three replications. The observed parameter was the diameter of the bacterial growth inhibition zone. The results showed that the methanolic extract at concentrations of 25%, 50%, and 75%, positive control ciprofloxacin, and negative control 1% CMC produced inhibition zone diameters of 6.35 ± 0.67 mm, 8.53 ± 0.79 mm, 11.23 ± 1.09 mm, 26.53 ± 0.92 mm, and 0.00 ± 0.00 mm, respectively. One-way ANOVA analysis revealed a significant difference among treatments (P < 0.05). In conclusion, the methanolic extract of ant plant tubers (Myrmecodia tuberosa) exhibits antibacterial activity against Shigella sp. Keywords: Antibacterial activity, disc diffusion, inhibition zone, Myrmecodia tuberosa, Shigella sp.