Electronic Theses and Dissertation

Escherichia coli tergolong bakteri Gram negatif yang secara normal terdapat di saluran pencernaan manusia maupun hewan, namun beberapa strain Escherchia coli bersifat patogen dan mampu menimbulkan penyakit kolibasilosis serta bersifat zoonosis. Penelitian ini bertujuan untuk mengisolasi bakteri Escherichia coli patogen pada burung gereja (Passer montanus). Penelitian ini menggunakan metode observasi dengan pendekatan cross sectional study. Sebanyak 22 sampel swab kloaka diisolasi dengan menggunakan media Eosin Methylen Blue Agar (EMBA) serta Sorbitol MacConkey Agar (SMAC). Proses isolasi dilakukan menggunakan metode Carter (1987) dan diinkubasi pada suhu 37°C selama 24 jam. Koloni yang berwarna hijau metalik pada EMBA diidentifikasi sebagai Escherichia coli, sedangkan koloni tidak berwarna (colourless) pada SMAC menunjukkan strain Escherichia coli patogen. Hasil penelitian menunjukkan bahwa sebanyak 16 dari 22 sampel (72,7%) dinyatakan positif mengandung bakteri Escherichia coli patogen. Hasil ini mengindikasikan bahwa burung gereja berpotensi menjadi reservoir dan sumber penyebaran Escherichia coli patogen di lingkungan sekitar kandang unggas, sehingga perlu dilakukan pengendalian dan penerapan biosekuriti untuk mencegah penularan ke manusia maupun hewan lain.

Escherichia coli is a Gram-negative bacterium that normally inhabits the digestive tract of humans and animals; however, several strains of Escherichia coli are pathogenic and capable of causing colibacillosis as well as being zoonotic in nature. This study aimed to isolate pathogenic Escherichia coli from house sparrows (Passer montanus). The study employed an observational method with a cross-sectional study approach. A total of 22 cloacal swab samples were isolated using Eosin Methylene Blue Agar (EMBA) and Sorbitol MacConkey Agar (SMAC). The isolation process was carried out using the Carter (1987) method and incubated at 37°C for 24 hours. Colonies showing a metallic green color on EMBA were identified as Escherichia coli, while colorless colonies on SMAC indicated pathogenic strains of Escherichia coli. The results showed that 16 out of 22 samples (72.7%) were positive for pathogenic Escherichia coli. These findings indicate that house sparrows have the potential to act as reservoirs and sources of transmission of pathogenic Escherichia coli in the environment surrounding poultry farms; therefore, control measures and the implementation of biosecurity are necessary to prevent transmission to humans and other animals.