Electronic Theses and Dissertation

EFEK PENAMBAHAN LIMBAH IKAN TERHADAP ANTIOKSIDAN PROBIOTIK RAGI DAN BAKTERI ASAM LAKTAT (RABAL)
Antioksidan merupakan senyawa yang mampu menghambat reaksi radikal bebas dalam tubuh. Bakteri asam laktat (BAL) sebagai probiotik adalah kelompok bakteri yang menghasilkan asam laktat sebagai produk utama dalam proses fermentasi. Bakteri asam laktat (BAL) menghasilkan metabolit selama fermentasi seperti asam organik, asam amino, vitamin, eksopolisakarida diantaranya memiliki efek meningkatkan kesehatan seperti antioksidan. Penelitian bertujuan mengetahui pengaruh penambahan limbah ikan antioksidan probiotik ragi dan bakteri asam laktat (RABAL). Penelitian ini menggunakan lima perlakuan P0+ (asam askorbat), P0−(RABAL tanpa penambahan limbah ikan), P1 (RABAL ditambah 10% limbah ikan), P2 (RABAL ditambah 20% limbah ikan), dan P3 (RABAL ditambah 30% limbah ikan). Uji antioksidan dilakukan dengan menggunakan metode DPPH (2,2-diphenyl-1-picrylhidrazil). Hasil penelitian berdasarkan nilai IC50 bahwa antioksidan pada probiotik RABAL P0+, P0−, P1, P2 dan P3 masing-masing adalah 2,33 μg/mL, 13,82 μg/mL, 7,08 μg/mL, 8,20 μg/mL, 8,71 μg/mL. Berdasarkan hasil penelitian dapat disimpulkan bahwa probiotik RABAL yang ditambah 10% limbah ikan (P1) memiliki antioksidan terbesar. Kata kunci: Antioksidan; IC50; limbah ikan; probiotik RABAL; radikal bebas.

EFFECT OF FISH WASTE ADDITION ON THE ANTIOXIDANT ACTIVITY OF PROBIOTIK YEAST AND LACTIT ACID BACTERIA (RABAL) Antioxidants are compounds that inhibit free radical reactions in the body. Lactic acid bacteria (LAB), which are probiotics, produce lactic acid as the main by product of fermentation. These bacteria produce metabolites such as organic acids, amino acids, vitamins, and exopolysaccharides during fermentation. Some of these metabolites have health-promoting effects, such as acting as antioxidants. The aim of this study is to determine the effect of adding antioxidant probiotic yeast and lactic acid bacteria (RABAL) to fish waste. Five treatments were used in this study: P0 (ascorbic acid), P0 (RABAL without fish waste addition), P1 (RABAL plus 10% fish waste), P2 (RABAL plus 20% fish waste), and P3 (RABAL plus 30% fish waste). The antioxidant test was conducted using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method. Based on the IC50 value, the results of the study showed that the antioxidant content of probiotic RABAL P0+ , P0- , P1, P2, and P3 was 2.33 μg/mL, 13.82 μg/mL, 7.08 μg/mL, 8.20 μg/mL, and 8.71 μg/mL, respectively. The results indicate that RABAL probiotics supplemented with 10% fish waste (P1) have the highest antioxidant content. Keywords: Antioxidant; free radical; Fish waste; IC50; probiotic RABAL.