Electronic Theses and Dissertation

Aeromonas hydrophila merupakan bakteri Gram negatif yang dapat menyebabkan penyakit Motil Aeromonas Septicemia (MAS) pada ikan nila, dengan tingkat kematian hingga 80- 100%. Kematian ikan pada kolam seringkali terjadi di lokasi Aceh Fisheries, Kabupaten Aceh Besar, dengan gejala bercak merah pada tubuh ikan. Penelitian ini bertujuan untuk mendeteksi cemaran bakteri Aeromonas hydrophila pada kolam ikan nila di lokasi Aceh fisheries, Aceh Besar. Penelitian ini menggunakan 1 sampel air yang berasal dari kolam bibit, 1 sampel air kolam indukan, 2 sampel air kolam pembesaran, 2 sampel air kolam panen serta 3 sampel ikan yang mati dan ikan yang sakit dari kolam panen. Hasil penelitian menunjukkan bahwa bakteri Aeromonas hydrophila dapat terdeteksi pada sampel kolam bioflok pembesaran 2, kolam beton panen 1, ikan 1 dan ikan 2. Berdasarkan hasil pemeriksaan, ditemukan adanya cemaran Aeromonas hydrophila pada sampel air dan ikan yang diambil dari kolam panen. Berdasarkan hasil penelitian dapat disimpulkan bahwa air kolam ikan nila di lokasi Aceh Fisheries tercemar oleh bakteri A. hydrophila.
Kata kunci: Aeromonas hydrophila, air kolam, cemaran bakteri, ikan nila

Aeromonas hydrophila is a Gram negative bacterium that causes Motile Aeromonas Septicemia (MAS) in Nile tilapia (Oreochromis niloticus), with a mortality rate of up to 80–100%. This study aims to detect the presence of A. hydrophila contamination in pond water and fish at the Aceh Fisheries site in Aceh Besar. A total of nine samples were collected, including water samples from the hatchery pond 1, broodstock pond 1, grow-out ponds 2, harvest ponds 2, and fish samples from diseased and dead tilapia in the harvest ponds 3. Bacterial isolation and identification were carried out through culture methods and biochemical tests. The results showed that A. hydrophila was detected in water samples from grow-out pond 2 (biofloc) and harvest pond 2 (concrete), as well as in two fish samples. These findings indicate that the pond environment, particularly the harvest ponds, is contaminated with A. hydrophila, which may contribute to disease outbreaks and fish mortality. Continuous monitoring of water quality and bacterial contamination is necessary to prevent further losses in tilapia farming. Keywords: Aeromonas hydrophila, pond water, bacterial contamination, Nile tilapia.