Electronic Theses and Dissertation

ISOLASI Escherichia coli PATOGEN PADA KUCING PENDERITA EAR MITES DI KECAMATAN BANDA RAYA

ABSTRAK


Escherichia coli merupakan bakteri Gram negatif yang bersifat patogen oportunistik apabila berada pada habitat yang tidak sesuai dan dalam jumlah yang banyak. Secara alami bakteri Escherichia coli merupakan flora normal dalam saluran pencernaan. Penelitian ini bertujuan untuk melakukan isolasi bakteri Escherichia coli sebagai penyebab infeksi sekunder pada kucing penderita ear mites. Sampel diambil dengan metode cross sectional study dengan jumlah sampel sebanyak 20 sampel yang diambil dari 10 desa di Kecamatan Banda Raya, Kota Banda Aceh. Isolasi dikerjakan berdasarkan Carter (1987). Escherichia coli diisolasi pada media Eosin Methylen Blue Agar (EMBA) menggunakan teknik goresan T dan diinkubasi pada temperature 37°C selama 24 jam hasil positif pada EMBA kemudian dilanjutkan dengan penanaman pada media Sorbitol-Mac Conkey Agar (SMAC) menggunakan teknik goresan T diinkubasi pada temperature 37°C selama 24 jam. Morfologi yang tumbuh kemudian diamati ukuran, bentuk, permukaan, tepi, elevasi dan aspek koloni. Bakteri yang tumbuh pada media EMBA akan menunjukkan warna hijau metalik, sedangkan bakteri yang tumbuh pada media SMAC tidak bewarna. Selanjutnya dilakukan pewarnaan Gram untuk mengelompokkan bakteri Gram negatif atau bakteri Gram positif. Morfologi yang tumbuh, kemudian diamati ukuran, bentuk, permukaan, tepi, elevasi dan aspek koloni. Hasil penelitian menunjukkan bahwa dari 20 sampel yang diperiksa terdapat 15 sampel positif Escherichia coli pada media EMBA, kemudian dari 15 sampel yang positif Escherichia coli dilanjutkan penanaman pada media SMAC, seluruhnya menunjukkan hasil positif Escherichia coli patogen. Berdasarkan hasil penelitian dapat disimpulkan bahwa 75% sampel kucinng penderita ear mites di Kecamatan Banda Raya positif bakteri Escherichia coli patogen.
Kata kunci: Ear mites, Escherichia coli, kucing, patogen

ISOLATION OF PATHOGENIC Escherichia coli IN CATS SUFFERING FROM EAR MITES IN THE SUB DISTRICT BANDA RAYA ABSTRACT Escherichia coli was a Gram-negative bacteria that acted as an opportunistic pathogen when in an unsuitable habitat and in large numbers. Naturally, Escherichia coli bacteria were normal flora in the digestive tract. This study aimed to isolate Escherichia coli bacteria as the cause of secondary infections in cats suffering from ear mites. Samples were taken using a cross-sectional study method, with a total of 20 samples collected from 10 villages in Banda Raya District, Banda Aceh City. Isolation was carried out based on Carter (1987). Escherichia coli was isolated on Eosin Methylene Blue Agar (EMBA) media using the T-streak technique and incubated at 37°C for 24 hours. Positive results on EMBA were then followed by plating on Sorbitol-Mac Conkey Agar (SMAC) media using the T-streak technique, and incubated at 37°C for 24 hours. The morphology that grew was then, observed for size, shape, surface, edge, elevation, and aspect of the colony. Bacteria that grew on EMBA media showed a metallic green color, while bacteria that grew on SMAC media were colorless. Furthermore, Gram staining was carried out to classify the bacteria as either Gram-negative or Gram-positive. The morphology was again observed for size, shape, surface, edge, elevation, and aspect of the colony. The results showed that out of 20 samples examined, there were 15 positive Escherichia coli samples on EMBA media, then from 15 samples that were positive for Escherichia coli, they were continued to be planted on SMAC media, all of which showed positive results for pathogenic Escherichia coli. Based on the research results, it could be concluded that 75% of the samples from cats suffering from ear mites in Banda Raya District were positive for pathogenic Escherichia coli bacteria. Keywords: Ear mites, Escherichia coli, cat, pathogen