Electronic Theses and Dissertation

UJI TOKSISITAS MENGGUNAKAN BRINE SHRIMP LETHALITY TEST (BSLT) PADA PROBIOTIK RAGI DAN BAKTERI ASAM LAKTAT (RABAL) SETELAH PENAMBAHAN LIMBAH IKAN
ABSTRAK
Untuk menentukan keamanan obat, suplemen, dan vitamin, uji pre klinis toksisitas dilakukan sebelum digunakan. Semua suplemen, termasuk probiotik, harus diuji untuk mengetahui apakah mereka berbahaya atau aman untuk dikonsumsi dalam dosis atau metode pemberian yang berbeda. Uji toksisitas Brine Shrimp Lethality Test (BSLT) digunakan untuk mengevaluasi keamanan senyawa kimia dan mengetahui bagaimana efek toksik suatu zat pada sistem biologi. Studi ini menyelidiki toksisitas probiotik RABAL ketika limbah ikan ditambahkan ke larva Artemia salina. Penelitian ini menggunakan rancangan acak lengkap (RAL), yang terdiri dari empat perlakuan: P0 sebagai kontrol (RABAL), P1 (RABAL ditambah 10% limbah ikan), P2 (RABAL ditambah 20% limbah ikan), dan P3 (RABAL ditambah 30% limbah ikan). Semua obat di fermentasi selama tujuh hari. Kemudian dibagi menjadi sepuluh konsentrasi, yaitu 2000; 1000; 500; 250; 125; 62,500; 31,250; 15,625; 7,812; dan 1,953 ppm. Persentase kematian larva Artemia salina dihitung sebelum menghitung nilai LC50 menggunakan analisis probit. Hasil penelitian menunjukkan bahwa penambahan limbah ikan meningkatkan toksisitas probiotik RABAL terhadap larva Artemia salina. Nilai P0 dan P1 masing-masing mencapai 1293,0628 g/ml dan 1041,2805 g/ml, masing-masing dengan nilai regresi y = 0,7866x + 2,5524 dan y = 0,7343x + 2,7842. Nilai P2 dan P3 masing-masing mencapai 807,0713 g/ml dan 737,3450 g/ml, masing- masing dengan Dengan dosis 1293,0628 g/ml dan 1041,2805 g/ml, probiotik RABAL dengan penambahan limbah ikan 10% dan 20% tidak berbahaya bagi larva Artemia salina.
Kata kunci: bakteri asam laktat, Brine Shrimp Lethality Test (BSLT), limbah ikan, probiotik, ragi, toksisitas

TOXICITY TEST USING BRINE SHRIMP LETHALITY TEST (BSLT) ON PROBIOTIC YEAST AND LACTIC ACID BACTERIA (RABAL) AFTER ADDING FISH WASTE ABSTRACT Preclinical toxicity testing is the first step in determining the safety levels of medications, vitamins, and supplements. Probiotics are supplements that must be tested for toxicity and safety at various dosages and administration methods. The Brine Shrimp Lethality Test (BSLT) is used to determine a substance's harmful effects on biological systems, gather distinctive dose-response data, and assess a chemical compound's safety. By adding fish waste, this study seeks to ascertain the toxicity value of RABAL probiotics against Artemia salina larvae. Four treatments—P0, the control (RABAL), P1 (RABAL + 10% fish waste), P2 (RABAL + 20% fish waste), and P3 (RABAL + 30% fish waste)—were included in the study's completely randomized design (CRD). The treatments were then separated into 10 concentrations (2000, 1000, 500, 250, 125, 62.5, 31.25, 15.625, 7.812, and 1.953 ppm) and fermented for seven days. The Artemia salina larval mortality percentage was used to measure the BSLT test, and probit analysis was used to get the LC50 value. With regression values of y = 0.7866x + 2.5524 and y = 0.7343x + 2.7842, respectively, the results demonstrated that the toxicity values of RABAL probiotics when fish waste was added to Artemia salina larvae were 1293.0628 μg/ml and 1041.2805 μg/ml for P0 and P1, respectively. In contrast, the regression values for P2 and P3 were y = 0.7176x + 2.914 and y = 0.6839x + 3.0388, respectively, and the values were 807.0713 μg/ml and 737.3450 μg/ml. For Artemia salina larvae, RABAL probiotics that added 20% and 30% fish waste were hazardous. The findings showed that at concentrations of 1293.0628 μg/ml and 1041.2805 μg/ml, RABAL probiotics without fish waste and with 10% fish waste were not harmful. Keywords: Brine Shrimp Lethality Test (BSLT), fish waste, lactic acid bacteria, probiotics, toxicity, yeast