Electronic Theses and Dissertation

Cue (Faunus ater) merupakan salah satu bahan baku yang dimanfaatkan masyarakat sebagai makanan khas daerah Aceh. Penelitian ini bertujuan untuk mengindentifikasi bakteri Shewanella algae pada cue (Faunus ater) menggunakan analisis gen 16S rRNA. Metode penelitian dilaksanakan secara observasi lapangan dan explorasi laboratorium menggunakan metode Carter yang dimodifikasi dan analisis gen 16S rRNA. Hasil penelitian menunjukkan secara metode Carter adanya pertumbuhan bakteri pada media NB yang ditandai dengan media menjadi keruh, terdapat koloni bewarna hitam pada media TCBS, pewarnaan Gram menunjukkan bakteri Gram negatif dan berbentuk basil. Hasil analisis menggunakan gen 16S rRNA menunjukkan sampel MZ-05 memiliki kemiripan dengan bakteri Shewanella algae dengan tingkat similarity 98,72%. Dapat disimpulkan bahwa, pada cue (Faunus ater) yang terdapat di Sungai Leupung, Sungai Saney dan Sungai Glee Bruek ditemukan adanya bakteri Shewanella algae berdasarkan analisis gen 16S rRNA.

Cue (Faunus ater) is one of the raw materials used by the community as a typical Acehnese food. This study aims to identify Shewanella algae bacteria in cue (Faunus ater) using 16S rRNA gene analysis. The research method was carried out by field observation and laboratory exploration using the modified Carter method and 16S rRNA gene analysis. The results showed by Carter method that there was bacterial growth on NB media which was marked by the media becoming cloudy, there were black colonies on TCBS media, Gram staining showed Gram negative and bacillus-shaped bacteria. The results of the analysis using the 16S rRNA gene showed that sample MZ-05 was similar to Shewanella algae with a similarity level of 98.72%. It can be concluded that, in cue (Faunus ater) found in Leupung River, Saney River and Glee Bruek River, the presence of Shewanella algae bacteria was found based on 16S rRNA gene analysis.