Electronic Theses and Dissertation

Kolagen tulang ikan tuna sirip kuning dan red palm oil dikembangkan menjadi produk antiaging dalam bentuk ampul kosmetik. Penelitian ini dilakukan dengan tujuan mengkarakterisasi red palm oil, minyak nilam fraksi ringan, dan virgin coconut oil serta diformulasikannya ampul kosmetik dengan kandungan kolagen tulang ikan tuna sirip kuning dan red palm oil yang memenuhi persyaratan evaluasi, stabilitas, keamanan, dan efektivitas. Karakterisasi meliputi berat jenis, indeks bias, dan analisis senyawa dengan GCMS. Sediaan dievaluasi organoleptis, homogenitas, pH, viskositas, sifat alir, serta keamanan cemaran mikroba dan logam beratnya. Stabilitas diuji dengan metode cycling test. Uji aktivitas antioksidan sediaan dilakukan dengan metode DPPH. Hasil karakterisasi indeks bias dan berat jenis telah berada dalam rentang yang disyaratkan. Hasil uji GCMS telah menunjukkan bahwa red palm oil dan minyak nilam fraksi ringan mengandung senyawa yang umum terdapat dalam masing-masing minyak tersebut. Ampul dibuat dengan 0,1% kolagen tulang ikan tuna sirip kuning serta red palm oil 0% (F0), 15% (F1), dan 25% (F2). Dihasilkan ampul berwarna putih keruh untuk F0 dan jingga untuk F1 dan F2. Terdapat aroma khas minyak kelapa dengan tekstur agak cair hingga cair dan homogen baik sebelum maupun setelah uji stabilitas pada ketiga formulasi. Selama masa pengujian, pH berada dalam rentang 5,37 – 6,03, viskositas dalam rentang 94,89 – 258,70 cPoise, sifat alir pseudoplastis, serta aman dari cemaran mikroba dan logam berat. Aktivitas antioksidan tergolong kuat pada F0 dan sangat kuat pada F1 dan F2. Kesimpulannya, minyak-minyak yang digunakan dalam formulasi telah memenuhi standar serta ampul kosmetik antiaging dengan kandungan kolagen tulang ikan tuna sirip kuning dan red palm oil juga telah memenuhi kriteria evaluasi, stabilitas, keamanan, dan memiliki aktivitas antioksidan kuat hingga sangat kuat sehingga punya potensi sebagai antiaging.

Yellowfin tuna bone collagen and red palm oil were developed into antiaging product in the form of cosmetic ampoules. This study was conducted with the aim of characterizing red palm oil, light fraction patchouli oil, and virgin coconut oil and formulating cosmetic ampoules containing yellowfin tuna bone collagen and red palm oil that meet the requirements of evaluation, stability, safety, and effectiveness. Characterization included specific gravity, refractive index, and compound analysis by GCMS. The preparation was evaluated for organoleptics, homogeneity, pH, viscosity, flow properties, and safety of microbial contamination and heavy metals. Stability was tested with the cycling test method. Antioxidant activity test of the preparation was carried out by DPPH method. The refractive index and specific gravity characterization results were within the required range. GCMS test results have shown that red palm oil and light fraction patchouli oil contain compounds that are common in each oil. Ampoules were made with 0.1% yellowfin tuna bone collagen and 0% (F0), 15% (F1), and 25% (F2) red palm oil. The resulting ampoules were cloudy white for F0 and orange for F1 and F2. There was a distinctive aroma of coconut oil with a slightly liquid to liquid and homogeneous texture both before and after the stability test in all three formulations. During the test period, the pH was within the range of 5.37 - 6.03, viscosity within the range of 94.89 - 258.70 cPoise, pseudoplastic flow properties, and safe from microbial and heavy metal contamination. Antioxidant activity was classified as strong in F0 and very strong in F1 and F2. In conclusion, the oils used in the formulation have met the standards and the antiaging cosmetic ampoules containing yellowfin tuna bone collagen and red palm oil have also met the evaluation criteria, stability, safety, and have strong to very strong antioxidant activity so that they have potential as antiaging.