Electronic Theses and Dissertation

ABSTRAK
Inflamasi dan stres oksidatif berperan pada aterogenesis. Pada keadaan stres oksidatif, dihasilkan LDL teroksidasi (Ox-LDL). Hal ini perlu dinetralisir dengan antioksidan. N-Asetil Sistein (NAC) mengandung glutathione, suatu zat antioksidan. Kami meneliti efek NAC pada tahap awal aterosklerosis.
Penelitian eksperimental dilakukan di Laboratorium Prof. Noerjanto Rumah Sakit Hewan Pendidikan Universitas Syiah Kuala. Sebanyak tiga puluh ekor tikus Rattus-Novergicus galur Wistar dibagi menjadi 6 kelompok yaitu kontrol negatif, kontrol positif, kelompok perlakuan NAC 600mg dan kelompok perlakuan 1200mg selama 2 minggu dan 6 minggu (N, DL, DLN6-2, DLN6-6, DLN12-2, dan DLN12-6).
Sebelum pemberian NAC, tikus diinduksi menjadi aterogenik selama 8 minggu dengan diet tinggi lemak. Setelah intervensi selesai, kami mengambil serum Ox-LDL dan leukosit dari sampel darah. Kami menggunakan ANOVA Faktorial, LSD post hoc, dan uji korelasi untuk menganalisis data.
NLR tidak berbeda signifikan secara statistik antar kelompok (nilai p > 0,05). Kadar Ox-LDL antara tikus aterogenik dengan kelompok kontrol negatif berbeda secara signifikan (p value

ABSTRACT Inflammation and oxidative stress were involved in atherogenesis. Under oxidative stress state, oxidized LDL (Ox-LDL) was formed. It needs neutralized by antioxidant. N-Acetyl Cysteine (NAC) contains glutathione, the antioxidant substances. We investigated the beneficial effect of NAC in early stage of atherosclerosis. An experimental study was conducted in Laboratory of Prof. Noerjanto Hospital Teaching Veterinary Universitas Syiah Kuala. Thirty male Rattus-Novergicus Wistar strain mice were divided to 6 groups which were negative control, positive control, NAC 600mg treatment and 1200mg treatment groups for 2 weeks and 6 weeks (N, DL, DLN6-2, DLN6-6, DLN12-2, and DLN12-6 subsequently). Before giving NAC, we induced mice to be atherogenic state for 8 weeks duration using high fat diet (HFD). After completing the intervention, we collected serum Ox-LDL and leucocyte from blood samples. We use Factorial ANOVA, LSD post hoc, and correlation test to analyse the data. The NLR was not statiscally significant difference between groups (p value > 0.05). The Ox-LDL level between atherogenic mice and control negative group was significantly difference (p value < 0,05). The highest and lowest Ox-LDL was found in positive control group (348,96 ng/mL) and in the 2 groups given NAC for 6 weeks (DLN6-6 and DLN12-6 with Ox-LDL level were 148,37 ng/mL and 154,18 ng/mL, respectively). Duration of treatment was significantly difference (DLN6-6 vs DLN6-2, DLN12-6 vs DLN 12-2, p value