Electronic Theses and Dissertation

Endometritis merupakan peradangan pada lapisan endometrium uterus yang disebabkan
karena infeksi bakteri, terutama melalui vagina dan menembus serviks. Pengobatan endometritis
dapat dilakukan dengan pemberian antibiotik, tetapi penggunaan antibiotik dapat menyebabkan
resistensi. Bawang putih memiliki kandungan metabolit sekunder yaitu alliin dan alliinase yang
diyakini dapat mengobati endometritis. Tujuan penelitian ini mengetahui efek pemberian ekstrak
bawang putih terhadap gambaran histopatologi tikus putih model endometritis. Penelitian ini
menggunakan rancangan acak lengkap dengan 6 perlakuan dan 3 ulangan. Tikus yang digunakan
sebanyak 18 ekor dan diinjeksi hormon progesteron selama 5 hari. P0 diinjeksikan NaCl fisiologis
sebanyak 0,1 ml tanpa diinduksi kombinasi bakteri; P1 diinduksi kombinasi bakteri E. coli dan S.
aureus dengan konsentrasi 1,5 x 10
8
CFU/ml; P2 diinduksi bakteri dan diinjeksikan kloramfenikol
dengan dosis 315 mg/kg BB; P3 diinduksi bakteri dan diberikan ekstrak bawang putih dengan dosis
250 mg/200 g BB; P4 diinduksi bakteri dan diberikan ekstrak bawang putih dengan dosis 500
mg/200 g BB; dan P5 sebagai kelompok yang diinduksi bakteri dan diberikan ekstrak bawang putih
dengan dosis 1000 mg/200 g BB. Perlakuan diberikan selama 7 hari. Setelah itu organ uterus tikus
putih diambil untuk dibuat preparat histopatologi. Parameter pada penelitian ini adalah perubahan
bentuk lumen, nekrosis sel epitel, proliferasi sel epitel serta temuan histopatologis lainnya seperti
sel radang, hiperemi dan hemoragi. Data bentuk lumen uterus disajikan secara deskriptif.
Sedangkan, data nekrosis dan proliferasi sel epitel dianalisis menggunakan uji Kruskal-Wallis
dilanjutkan dengan uji Mann-Whitney. Hasil uji statistik menunjukkan adanya penurunan jumlah
nekrosis dan proliferasi sel epitel (P

Endometritis is an inflammation of the endometrial lining of the uterus caused by bacterial infection, typically entering through the vagina and cervix. While antibiotics are commonly used to treat endometritis, their use can lead to antibiotic resistance. Garlic contains secondary metabolites, such as alliin and alliinase, which are believed to have therapeutic effects on endometritis. This study aimed to assess the impact of garlic extract on the histopathological features of a white rat model of endometritis. The study employed a completely randomized design with 6 treatments and 3 replicates, using 18 mice that were injecting progesterone hormone for 5 days. Group P0 was injected with 0.1 ml of physiological NaCl without bacterial induction; P1 was induced with a bacterial combination of E. coli and S. aureus at a concentration of 1.5 x 10^8 CFU/ml; P2 was induced with bacteria and injected with chloramphenicol at a dose of 315 mg/kg BW; P3 was induced with bacteria and given garlic extract at a dose of 250 mg/200 g BW; P4 was induced with bacteria and given garlic extract at a dose of 500 mg/200 g BW; and P5 was induced with bacteria and given garlic extract at a dose of 1000 mg/200 g BW. Treatments were administered for 7 days, after which the uterine organs of the white rats were collected to prepare histopathological slides. The study's parameters included changes in lumen shape, epithelial cell necrosis, epithelial cell proliferation, and other histopathological such as inflammatory cells, hyperemia and hemorrhage. Data on the shape of the uterine lumen were presented descriptively, while necrosis and proliferation data were analyzed using the Kruskal-Wallis test followed by the Mann-Whitney test. Statistical test results showed a decrease in the number of necrosis and proliferation of epithelial cells (P < 0.05) between the treatment group and the negative control group. It can be concluded that the administration of garlic extract shows an improvement in the composition of the endometrial epithelium in white rat endometritis model. Keywords: Garlic, endometritis, necrosis, proliferation