Electronic Theses and Dissertation

Kulit udang adalah salah satu bahan baku yang menghasilkan kitin dan memiliki kandungan kitin sekitar 15% - 30%. Kitosan berfungsi sebagai antimikroba, karena kitosan memiliki enzim lisozim dan gugus aminopolisakarida yang mampu
menghambat pertumbuhan mikroba. Kitosan tersulfonasi memiliki sifat antibakteri yang kuat terhadap Gram-negatif. Porphyromonas gingivalis adalah bakteri penyebab terjadinya penyakit periodontal dan dominan pada penyakit periodontitis kronis yang terdapat di plak subgingiva. Tujuan penelitian untuk mengetahui pengaruh kitosan tersulfonasi dari kulit udang Vaname perairan Aceh yang berpengaruh terhadap pertumbuhan Porphyromonas gingivalis. Penelitian ini merupakan penelitian eksperimental laboratoris menggunakan post-test only control group design dengan perlakuan kitosan tersulfonasi konsentrasi 1%, 3%, 5%, dan 7%, antibiotik metronidazole sebagai kontrol positif dan akuades sebagai
kontrol negatif. Hasil uji FTIR menunjukkan kitosan telah berhasil tersulfonasi dan diperoleh hasil derajat deasetilasi sebesar 47,62%. Hasil rata-rata pengukuran diameter zona hambat menggunakan metode sumuran pada kontrol positif
metronidazole sebesar 24 mm, sedangkan kontrol negatif berupa akuades tidak menunjukkan adanya zona hambat, pada kelompok konsentrasi 1% sebesar 4,15 mm, konsentrasi 3% sebesar 6,87 mm, konsentrasi 5% sebesar 7,08 mm dan konsentrasi 7% sebesar 6,08 mm. Hasil uji Kruskal-Wallis menunjukkan terdapatnya perbedaan yang bermakna dari hasil pengukuran diameter zona hambat kitosan tersulfonasi terhadap pertumbuhan Porphyromonas gingivalis. Dapat disimpulkan bahwa kitosan tersulfonasi dari kulit udang Vaname (Litopenaeus vannamei) perairan Aceh pada konsentrasi 1%, 3%, 5%, dan 7% mampu menghambat pertumbuhan Porphyromonas gingivalis.

Shrimp shells are one of the raw materials that produce chitin and have a chitin content of around 15% - 30%. Chitosan functions as an antimicrobial, because chitosan has the enzyme lysozyme and aminopolysaccharide groups which can inhibit microbial growth. Sulfonated chitosan has strong antibacterial properties against Gram-negatives. Porphyromonas gingivalis is a bacteria that causes periodontal disease and is dominant in chronic periodontitis found in subgingival plaque. The aim of the research was to determine the effect of sulfonated chitosan from Vaname shrimp shells in Aceh waters on the growth of Porphyromonas gingivalis. This research is a laboratory experimental study using a post-test only control group design with sulfonated chitosan treatment at concentrations of 1%, 3%, 5%, and 7%, the antibiotic metronidazole as a positive control and distilled water as a negative control. The FTIR test results showed that the chitosan had been successfully sulfonated and a deacetylation degree of 47.62% was obtained. The average results of measuring the diameter of the inhibition zone using the well method in the positive control metronidazole were 24 mm, while the negative control in the form of distilled water did not show any inhibition zone, in the 1% concentration group it was 4.15 mm, the 3% concentration was 6.87 mm, 5% concentration is 7.08 mm and 7% concentration is 6.08 mm. The results of the Kruskal-Wallis test showed that there was a significant difference in the results of measuring the diameter of the inhibitory zone of sulfonated chitosan on the growth of Porphyromonas gingivalis. It can be concluded that sulfonated chitosan from the shell of Vaname shrimp (Litopenaeus vannamei) in Aceh waters at concentrations of 1%, 3%, 5% and 7% is able to inhibit the growth of Porphyromonas gingivalis.