Electronic Theses and Dissertation

Kanker paru-paru merupakan kanker paling mematikan di dunia. Pencarian obat untuk penyembuhan kanker paru-paru sangat banyak dilakukan sampai pada tanaman herbal. Nilam aceh Pogostemon cablin Benth., yang merupakan salah satu tanaman endemik Aceh, telah diteliti memiliki khasiat yang sangat banyak seperti antiinflamasi, antioksidan, antimikroba dan antidepresan. Selain itu dia memiliki sifat antikanker namun belum banyak penelitian yang menghubungkannya dengan kanker paru-paru. Penelitian ini bertujuan mengeksplorasi potensi dari senyawa Pogostemon cablin Benth., sebagai obat antikanker paru-paru. Analisis potensi ini dilakukan dengan pendekatan network pharmacology. Senyawa-senyawa tanaman Pogostemon cablin Benth., didapatkan dari data GCMS dan protein target kanker paru-paru didapatkan dari OMIM, GeneCard, serta DisGeNet. Membangun jaringan interaksi pada irisan protein target dibangun dengan basis data STRING, kemudian dimasukkan ke dalam aplikasi cytoscape untuk dianalisis pada jaringannya. Untuk analisis pathway menggunakan basis data metascape dan enrichr. Jaringan interaksi antar protein yang dibangun pada basis data STRING sebanyak 177 node dengan 238 edge, kemudian diperkecil dengan clustering menggunakan MCODE sehingga didapatkan 9 protein target. Hasil analisis KEGG dan GO pada 9 protein target menghasilkan mekanisme multi target “senyawa obat-protein target-Pathway” dimana senyawa 2,3,3-Trimethyl-2(3-methyl-buta-1,3-dienyl)- cyclohexanone, apo-12- lycopenal, 4,8a- dimethyl-6(prop-1-en-2-yl)- 3,5,6,7,8,8a- hexahydronaphthalen-2 (1h)-one, Acetic acid,3hydroxy-6- isopropenyl-4,8a- dimethyl 1,2,3,5,6,7,8,8a- octahydronaphthalen -2-yl ester dan 2-(4a,8-Dimethyl- 1,2,3,4,4a,5,6,7-octahydro- naphthalen-2-yl)
-prop-2-en-1-ol menargertkan EGFR, PIK3CA, PIK3CB, PIK3CD, MET, KDR, IL6ST, IGF1R dan PTPN6 melalui jalur-jalur Non-small cell lung cancer, PI3K-Akt signaling pathway, Rap1 signaling pathway, Ras signaling pathway, dan JAK STAT signaling pathway. Penelitian ini menjelaskan tentang potensi senyawa nilam Aceh sebagai kemungkinan obat kanker paru-paru, melalui mekanisme multi-target.

Lung cancer is the deadliest cancer in the world. The search for a cure for lung cancer has gone to develope herbal plants as potential drugs. Patchouli Aceh (Pogostemon cablin Benth.), which is one of the endemic plants of Aceh, has been studied to have many therapeutic properties such as anti-inflammatory, antioxidant, antimicrobial and antidepressant. Besides, he has anti-cancer properties but not much research has linked it to treatment of lung cancer. The study aims to explore the potential of the compound Pogostemon cablin Benth as a drug candidate against lung cancer. The plant compounds of Pogostemon cablin Benth are obtained from GCMS data and the protein target of lung cancer is from OMIM, GeneCard, and DisGeNet database. The Protein-Protein Interactions (PPI) between the targets is constructed on the STRING database, found of 177 nodes with 238 edges. Then continued to shrunk the network by clustering it using the MCODE algorithm and end up to get 9 target proteins. The results of KEGG and GO analysis on the 9 target protein results in a multi-target mechanism of “compound-protein target-Pathway" where the compounds which is 2,3,3-Trimethyl-2-(3-methyl-buta-1,3-dienyl)-cyclohexanone, apo-12-lycopenal, 4,8adimethyl-6-(prop-1-en-2-yl)-3,5,6,7,8,8a- hexahydronaphthalen-2(1h)-one, Acetic acid, 3-hydroxy-6- isopropenyl-4,8a-dimethyl 1,2,3,5,6,7,8,8a- octahydronaphthalen-2-yl ester and 2-(4a,8-Dimethyl-1,2,3,4,4a,5,6,7 -octahydro-naphthalen-2-yl) -prop-2-en1-ol targeting the protein targets such as EGFR, PIK3CA, PIK3CB, PIK3CD, MET, KDR, IL6ST, IGF1R and PTPN6 with the lung signaling pathway through Non-small cell lung cancer, PI3K-Akt signaling pathway, Rap1 signaling pathway, Ras signaling pathway, dan JAK STAT signaling pathway. So, thhis research purpose to discover and explains the potential of the Aceh patchouli compound as a possible cure for lung cancer, through a multi-target mechanism.