Electronic Theses and Dissertation

Hiperglikemia merupakan salah satu penyebab perkembangan spermatogenesis terhambat. Penelitian ini bertujuan mengetahui pengaruh peningkatan jumlah sel-sel spermatogenik dan penurunan jumlah sel leydig yang mengalami nekrosis pada jaringan testis tikus yang diinduksi aloksan setelah pemberian cuka buah senggani (Melastoma affine D.Don). Penelitian ini menggunakan metode Experimental Laboratory dengan pola Rancangan Acak Lengkap menggunakan 16 ekor tikus jantan (Rattus norvegicus L.) dibagi dalam 4 perlakuan normal (N), diberi aloksan (KN), diberi aloksan + glibenklamid (KP), diberi aloksan + cuka buah senggani 2 ml/kg BB (PC). Parameter yang diamati meliputi jumlah sel spermatogenik (spermatogonium, spermatosit, spermatid, sperma) dan jumlah sel leydig yang mengalami nekrosis. Data dianalisis dengan Analisis Varian (ANAVA) dan uji lanjut BNJ dengan taraf signifikan 0,01. Hasil penelitian menunjukkan bahwa terjadi penurunan pada sel spermatogenik perlakuan KN (610, 1311, 1061, 800) dibandingkan perlakuan N (867, 2366, 4966, 5773). Jumlah sel spermatogenik perlakuan PC (838, 2321,5, 4894,8, 5730), lebih baik dari perlakuan KP (758,8, 1977,5, 3674, 5073) dan hampir menyamai perlakuan N. Jumlah sel leydig yang mengalami nekrosis paling banyak terdapat pada perlakuan KN (36), diikuti KP (29,5), PC (5,5), N (1). Simpulan dari penelitian ini adalah pemberian cuka buah senggani sangat berpengaruh terhadap peningkatan jumlah sel spermatogenik dan penurunan jumlah sel leydig yang mengalami nekrosis pada testis tikus putih hiperglikemia.
Kata kunci: cuka, hiperglikemia, senggani, testis.

Hyperglycemia is one of the causes of hampered spermatogenesis development. This study aims to determine the effect of increasing the number of spermatogenic cells and decreasing the number of Leydig cells that experience necrosis in rat testicular tissue induced by alloxan after administration of senggani fruit vinegar (Melastoma affine D.Don). This research used the Experimental Laboratory method with a Completely Randomized Design pattern using 16 male rats (Rattus norvegicus L.) divided into 4 treatments: normal (N), given alloxan (KN), given alloxan + glibenclamide (KP), given alloxan + fruit vinegar senggani 2 ml/kg BW (PC). Parameters observed included the number of spermatogenic cells (spermatogonia, spermatocytes, spermatids, sperm) and the number of Leydig cells that experienced necrosis. Data were analyzed using Analysis of Variance (ANOVA) and BNJ further test with a significance level of 0.01. The results showed that there was a decrease in spermatogenic cells in the KN treatment (610, 1311, 1061, 800) compared to the N treatment (867, 2366, 4966, 5773). The number of spermatogenic cells in the PC treatment (838, 2321.5, 4894.8, 5730), was better than the KP treatment (758.8, 1977.5, 3674, 5073) and almost equal to the N treatment. The number of Leydig cells that experienced necrosis was the highest. mostly found in the KN treatment (36), followed by KP (29.5), PC (5.5), N (1). The conclusion of this research is that administration of senggani fruit vinegar has a significant effect on increasing the number of spermatogenic cells and decreasing the number of Leydig cells that experience necrosis in the testicles of hyperglycemic white rats. Key words: vinegar, hyperglycemia, senggani, testicles.