Electronic Theses and Dissertation

Jaringan ikat kolagen tipe III merupakan salah satu tipe jaringan ikat kolagen yang terdapat pada otot dan diduga menjadi salah satu faktor penentu keempukan daging. Sebaran dan kandungan jaringan ikat kolagen tipe III pada otot sapi aceh belum pernah dilaporkan. Tujuan dari penelitian yaitu untuk mengidentifikasi sebaran dan kandungan jaringan ikat kolagen tipe III pada dua jenis otot yang berbeda pada sapi aceh. Sampel daging berasal dari otot longissimus dorsi dan semitendinosus yang berasal dari 3 ekor sapi aceh jantan dewasa dengan kisaran bobot badan 200-300 kg dan body condition score (BCS) 3. Sampel dibuat menjadi preparat histologi dan diwarnai dengan metode pewarnaan imunohistokimia. Sebaran jaringan ikat kolagen tipe III pada otot longissimus dorsi dan semitendinosus dianalisis secara kualitatif menggunakan mikroskop cahaya yang selanjutnya akan dikuantifikasi menggunakan software ImageJ dan disajikan dalam bentuk persentase luas area. Data persentase luas area dianalisis menggunakan independent T-test. Hasil penelitian menunjukkan bahwa jaringan ikat kolagen tipe III teridentifikasi pada endomisium dan perimisium kedua jenis otot. Hasil uji statistik memperlihatkan bahwa kandungan jaringan ikat kolagen tipe III pada otot longissimus dorsi dan semitendinosus berbeda nyata (p

Type III collagen connective tissue is one type of collagen connective tissue found in muscles and is thought to be one of the determining factors for meat tenderness. The distribution and content of type III collagen connective tissue in aceh cattle muscle has never been reported. The purpose of this study was to identify the distribution and content of type III collagen connective tissue in two different types of muscles in aceh cattle. The meat samples were taken from the longissimus dorsi and semitendinosus muscles of 3 adult male aceh cattles with body weight range between 200-300 kg and body condition score (BCS) 3. Histological preparation was made of samples and stained by immunohistochemistry staining method. The distribution of type III collagen connective tissue in longissimus dorsi and semitendinosus muscles was analyzed qualitatively using light microscope which was then be quantified using ImageJ software and presented in the form of a percentage area. The percentage area of type III collagen connective tissue was analyzed using an independent T-test. The result showed that the distribution of type III collagen connective tissue was found in the endomysium and perimysium of both types of muscles. The results of statistical tests showed that the content of type III collagen connective tissue from longissimus dorsi and semitendinosus muscles was significantly different (p