Electronic Theses and Dissertation

ISOLASI DAN IDENTIFIKASI BAKTERI ASAM LAKTAT Lactobacillus sp. DARI FESES GAJAH SUMATERA (Elephas maximus sumatranus)

ABSTRAK

Bakteri asam laktat (BAL) tergolong jenis mikroflora probiotik yang dapat menghasilkan asam laktat terutama dari golongan Lactobacilli dan Bifidobacteria melalui proses fermentasi. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi Bakteri Asam Laktat (BAL) Lactobacillus sp. yang terdapat pada feses gajah sumatera (Elephas maximus sumatranus) di Pusat Konservasi Gajah (PKG) Saree, Aceh Besar dan Conservation Response Unit (CRU) Sampoiniet, Aceh Jaya berdasarkan metode Carter. Sampel feses gajah sumatera diambil dari lima ekor gajah yang ada di PKG Saree, Aceh Besar dan tiga ekor gajah yang ada di CRU Sampoiniet, Aceh Jaya. Media selektif yang digunakan yaitu de Mann Rogosa Sharpe Agar (MRSA) dengan metode streak plate. Koloni yang tumbuh di media MRSA diamati morfologinya dan dilakukan pewarnaan Gram, uji katalase serta dilanjutkan dengan uji biokimia yaitu uji indol, Simmon’s Citrate Agar (SCA), Methyl Red-Voges Proskauer (MR-VP), Sulfit Indole Motility (SIM), Triple Sugar Iron Agar (TSIA), dan gula-gula. Hasil isolasi dan identifikasi terhadap delapan sampel feses gajah sumatera ditemukan Lactobacillus sp. pada tujuh sampel gajah sumatera (lima sampel dari PKG Saree, Aceh Besar dan dua sampel dari CRU Sampoiniet). Oleh sebab itu, dapat disimpulkan bahwa bakteri asam laktat Lactobacillus sp dapat diidentifikasi pada feses gajah sumatera (Elephas maximus sumatranus) di PKG Saree, Aceh Besar dan CRU Sampoiniet, Aceh Jaya.

Kata kunci: bakteri asam laktat, Lactobacillus sp., feses, gajah sumatera

ISOLATION AND IDENTIFICATION OF LACTID ACID BACTERIA Lactobacillus sp. FROM SUMATRAN ELEPHANTS (Elephas maximus sumatranus) FAECES ABSTRACT Lactic Acid Bacteria (LAB) are classified as a type of probiotic microflora that can produce lactic acid mainly from the class of Lactobacilli and Bifidobacteria through fermentation This study aimed to isolate and identify the Lactic Acid Bacteria (LAB) Lactobacillus sp. found in the feces of Sumatra elephant (Elephas maximus sumatranus) in Elephant Training Centre (ETC) Saree Aceh Besar and Conservation Response Unit (CRU) Sampoiniet Aceh Jaya based on Carter method. The Sample feces of sumatran elephant takes from five elephant in ETC Saree Aceh Besar and three elephant in CRU Sampoiniet Aceh Jaya. The selective media used were de Mann Rogosa Sharpe Agar (MRSA). Separated colonies which grown in MRSA media were observed for their morphologies, and continued by Gram staining, catalase test then continued with biochemical test such as indol test, Simmon’s Citrate Agar (SCA), Methyl Red-Voges Proskauer (VP), sulphites indole motility (SIM), triple sugar iron agar (TSIA), and sugar test. Lactobacillus sp. was identified in seven samples of sumatran elephants (five samples from ETC Saree, Aceh Besar and two samples from CRU Sampoiniet). In conclusion, the lactic acid bacteria Lactobacillus sp. was identified from the feces of sumatran elephant (Elephas maximus sumatranus) in ETC Saree, Aceh Besar and CRU Sampoiniet, Aceh Jaya. Keywords: lactic acid bacteria, lactobacillus sp., feces, sumatran elephant