Electronic Theses and Dissertation

ABSTRAK

Sebagai produk asal hewan, daging cukup rentan terkontaminasi mikroba seperti Escherichia coli, bakteri penyebab diare akut. Tujuan dilakukannya penelitian ini adalah untuk mengisolasi dan mengidentifikasi Escherichia coli yang mengontaminasi daging sapi yang dijual di Pasar Tradisional Kota Padang Panjang. Penelitian ini didasarkan pada metode pengambilan sampel cross sectional dengan metode pengerjaan di lab menggunakan Cowan and Steel’s (1974). Pengambilan sampel 10 daging sapi dengan berat 100 gram dilakukan pada pagi hari di Pasar Tradisional Kota Padang Panjang. Analisis data bersifat deskriptif. Data yang diperoleh dideskripsikan dengan menampilkan tabel dan foto hasil pengamatan. Hasil penelitian diperoleh total 10 sampel menunjukkan negatif Escherichia coli. Isolasi bakteri dilakukan pada media MacConkey Agar yang ditunjukkan dengan koloni berwarna merah muda pudar. Pengidentifikasian hasil negatif diperoleh berdasarkan uji biokimia (SIM, methyl-red, voges proskauer, urea, fenilalanin, lisin, ornitin, KCN, OF, gelatin, sitrat, TSIA, laktosa, glukosa). Berdasarkan data hasil penelitian disimpulkan bahwa tidak ditemukannya Escherichia coli pada 10 sampel daging sapi yang diambil dari Pasar Tradisional Kota Padang Panjang.

Kata kunci: Escherichia coli, diare, daging sapi

ABSTRACT As a product of animal origin, meat is quite susceptible to contamination by microbes such as Escherichia coli, a bacterium that causes acute diarrhea. The purpose of this research was to isolate and identify Escherichia coli that contaminates meat sold at the Padang Panjang Traditional Market. This research is based on the cross sectional sampling method with the method of working in the lab using Cowan and Steel's (1974). Sampling of 10 meat weighing 100 grams was carried out in the morning at the Padang Panjang Traditional Market. Data analysis is descriptive. The data obtained are described by displaying tables and photos of observations. The results obtained a total of 10 samples showed negative Escherichia coli. Bacterial isolation was carried out on MacConkey Agar media which was indicated by faded pink colonies. Identification of negative results obtained based on biochemical tests (SIM, methyl-red, voges proskauer, urea, phenylalanine, lysine, ornithine, KCN, OF, gelatin, citrate, TSIA, lactose, glucose). Based on the research to the data, it was concluded that Escherichia coli was not found in 10 meat samples taken from the Padang Panjang City Traditional Market. Keywords: Escherichia coli, diarrhea, meat.