Electronic Theses and Dissertation

Tanaman sirih merah (Piper crocatum) merupakan tanaman yang memiliki manfaat dalam
pengobatan berbagai penyakit. Penelitian ini bertujuan mengetahui aktivitas antibakteri daun sirih
merah yang diekstraksi dengan menggunakan pelarut n-heksana, etil asetat, dan etanol 96% terhadap
pertumbuhan bakteri Eschrichia coli secara in vitro. Penelitian ini menggunakan pelarut n-heksana,
etil asetat, dan etanol 96% serta 5 konsentrasi ekstrak sirih merah yaitu 0%, 10%, 20%, 40%, dan
60%. Kontrol positif menggunakan kloramfenikol. Uji aktivitas antibakteri dilakukan menggunakan
metode Kirby-Bauer. Data dianalisis secara deskriptif dan disajikan dalam bentuk tabel. Hasil
penelitian menunjukkan tidak terbentuk zona hambat di sekitar cakram disk pada ekstrak n-heksana
dan etanol 96%, sedangkan ekstrak etil asetat pada konsentrasi 10%, 20%, 40%, dan 60%
membentuk zona hambat dengan diameter masing-masing 8,97 ± 0,40; 11,61 ± 0,47; 13,4 ± 0,98;
17,56 ± 0,62. Ekstrak etil asetat memiliki aktivitas antibakteri yang mampu menghambat
pertumbuhan bakteri Escherichia coli, sedangkan ekstrak n-heksana dan etanol 96% tidak mampu
menghambat pertumbuhan bakteri Escherichia coli secara in vitro.

Red betel plant (Piper crocatum) is a plant that has benefits in the treatment of various diseases. This study aimed to determine the antibacterial activity of red betel leaf extracted using n- hexane, ethyl acetate, and 96% ethanol as solvents against the growth of Eschrichia coli in vitro. This study used n-hexane, ethyl acetate, and 96% ethanol as solvents and 5 concentrations of red betel extract, namely 0%, 10%, 20%, 40%, and 60%. Chloramphenicol disc was used as a positive control. Antibacterial activity test was carried out using Kirby-Bauer method. The data were analyzed descriptively and presented in tabular form. The results showed that no inhibition zone was formed around the discs containing n-hexane and 96% ethanol extract, while the ethyl acetate extract at concentrations of 10%, 20%, 40%, and 60% showed inhibition zone with average diameter were 8,97 ± 0,40; 11,61 ± 0,47; 13,4 ± 0,98; 17,56 ± 0,62, respectively. Ethyl acetate extract had antibacterial activity that was able to inhibit the growth of Escherichia coli, while n- hexane and 96% ethanol extracts were unable to inhibit the growth of Escherichia coli in vitro.