Electronic Theses and Dissertation

Modifikasi kitosan dengan asam amino yang diperoleh dari proses hidrolisis protein limbah cair tahu dan TiO2 telah dilakukan. Berdasarkan hasil analisis LCMS/MS diketahui bahwa asam amino yang terdapat pada sampel hidrolisat protein limbah cair tahu adalah tirosin, sistin, asam glutamat dan asam aspartat. Film kitosan-asam amino/TiO2 dikarakterisasi menggunakan XRD, FT-IR, TGA, DSC. SEM-EDX dan uji tarik. Berdasarkan hasil XRD, penambahan asam amino dan TiO2 sedikit mengubah sifat kristalinitas pada kitosan. Hasil FT-IR mengkonfirmasi terbentuknya film kitosan-asam amino/TiO2. Hasil TGA dan DSC menunjukan bahwa penambahan asam amino menurunkan stabilitas film terhadap termal, namun penambahan TiO2 mampu meningkatkan stabilitas film terhadap termal. Berdasarkan hasil uji tarik, penambahan asam amino dan TiO2 pada kitosan mampu meningkatkan sifat mekanik pada kitosan. Berdasarkan pengujian kinetika adsorpsi dan adsorpsi-fotodegradasi, penghilangan metilen biru mengikuti model pseudo second order. Hasil uji termodinamika adsorpsi dan adsorpsi-fotodegradasi menunjukkan bahwa proses adsorpsi bersifat endotermik sedangkan proses adsorpsi-fotodegradasi bersifat eksotermik. Model isotherm pada proses adsorpsi mengikuti model isotherm Freundlich dan diperoleh kapasitas serapan maksimum sebesar 4,693 mg/g sedangkan pada proses adsorpsi-fotodegradasi mengikuti model isotherm Langmuir dan dihasilkan kapasitas serapan maksimum sebesar 33,003 mg/g. Berdasarkan hasil uji regenerasi, film kitosan-asam amino/TiO2 dapat diregenerasi.

Kata Kunci: Kitosan, Asam Amino, Limbah Cair Tahu, TiO2, Metilen Biru

Modification of chitosan with amino acids obtained from the protein hydrolysis process of tofu wastewater and TiO2 has been carried out. Based on the results of the LCMS/MS analysis, it is known that the amino acids contained in the protein hydrolyzate from tofu wastewater are tyrosine, cystine, glutamic acid and aspartic acid. Chitosan-amino acid/TiO2 films were characterized using XRD, FT-IR, TGA, DSC, SEM-EDX and Tensile Strength. Based on the XRD results, the addition of amino acids and TiO2 slightly changed the crystallinity properties of chitosan. FT-IR results confirmed the formation of chitosan-amino acid/TiO2 films. The results of TGA and DSC showed that the addition of amino acids decreased the thermal stability of the film, but the addition of TiO2 was able to increase the thermal stability of the film. Based on the results of the tensile strength, the addition of amino acids and TiO2 to chitosan was able to improve the mechanical properties of chitosan. Based on the adsorption and adsorption-photodegradation kinetics, the removal of methylene blue followed a pseudo second order model. The thermodynamic results of adsorption and adsorption-photodegradation show that the adsorption process is endothermic while the adsorption-photodegradation process is exothermic. The isotherm model in the adsorption process follows the Freundlich isotherm model and the maximum absorption capacity is 4,693 mg / g, while the adsorption-photodegradation process follows the Langmuir isotherm model and the resulting maximum absorption capacity is 33,003 mg / g. Based on the regeneration test, the chitosan-amino acid/TiO2 films could be regenerated. Keywords: Chitosan, Amino Acid, Tofu Wastewater, TiO2, Methylene Blue