Electronic Theses and Dissertation

Penelitian ini bertujuan untuk mengetahui bagaimana pengaruh pemberian ekstrak etanol kulit batang Mezzetia parviflora (EEKBMP) terhadap perubahan struktur histologis hati tikus (Rattus norvegicus) setelah induksi parasetamol dosis toksik. Metode yang digunakan dalam penelitian ini adalah Rancangan Acak Lengkap (RAL) yang terdiri dari 6 perlakuan dan 4 ulangan. Hewan uji yang digunakan ialah tikus jantan galur Wistar usia 3 bulan dengan rerata berat badan 271,375 ± 15,251 g sebanyak 24 individu tikus. Perlakuan terdiri dari 3 kontrol yaitu kontrol normal (akuades), kontrol negatif (parasetamol 1.350 mg/kgBB dan akuades), kontrol positif (parasetamol dosis 1.350 mg/kgBB dan HEPA-Q 11,34 mg/kgBB) dan 3 pemberian ekstrak etanol kulit batang Mezzetia parviflora (EEKBMP) dengan dosis 150 mg/kgBB (P1), dosis 300 mg/kgBB (P2) dan dosis 600 mg/kgBB (P3). Seluruhnya diberikan perlakuan selama 14 hari dan diinduksi parasetamol pada hari ke-7 sebanyak 1.350 mg/kg BB kecuali pada kontrol normal (K0). Organ hati hewan uji dijadikan sediaan histologi dengan menggunakan metode paraffin dan pewarnaan Hematoksilin & Eosin (HE). Parameter yang diukur adalah jumlah proporsi sel hepatosit hati yang mengalami kerusakan berupa degenerasi dan nekrosis. Data yang diperoleh dianalisis menggunakan uji ANOVA dengan taraf signifikan 5% dan dilanjutkan dengan uji berganda Duncan. Hasil penelitian menunjukkan pemberian ekstrak etanol kulit batang Mezzetia parviflora (EEKBMP) berpengaruh nyata (P

This study aims to determine the effect of the ethanol extract of the bark of Mezzetia parviflora (EEKBMP) on changes in the histological structure of the liver of rats (Rattus norvegicus) after induction of toxic doses of paracetamol. The method used in this study was Completely Randomized (CRD) consisting of 6 treatments and 4 replications. The test used was male rats aged 3 months with an average body weight of 271.375 ± 15.251 g as many as 24 individual animals. The treatment consisted of 3 controls, namely normal control (aquadest), negative control (1,350 mg/kgBW paracetamol and distilled water), positive control (1,350 mg/kgBW paracetamol and 11,34 mg/kgBW HEPA-Q) and 3 administration of ethanol extract of the skin stems of Mezzetia parviflora (EEKBMP) at a dose of 150 mg/kgBW (P1), a dose of 300 mg/kgBW (P2) and a dose of 600 mg/kgBW (P3). All of them were treated for 14 days and induced by paracetamol on the 7th day as much as 1,350 mg/kg BW except for normal controls (K0). Liver organs of the test animals were made into histological preparations using the paraffin method and Hematoxylin & Eosin (HE) staining. The parameter measured was the proportion of liver hepatocyte cells that were damaged in the form of degeneration and necrosis. The data obtained were analyzed using ANOVA test with a significant level of 5% and continued with Duncan's multiple test. The results showed that the ethanol extract of the stem bark of Mezzetia parviflora (EEKBMP) had a significant effect (P