Electronic Theses and Dissertation

ABSTRAK Spons menghasilkan senyawa bioaktif yang berasal dari bakteri asosiasi yang berpotensi sebagai antimikroba. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi DNA bakteri asosiasi spons berdasarkan analisis gen 16S rRNA. Hasil Penelitian menunjukkan sebanyak delapan isolat bakteri asosiasi spons RBX-1, RBX-2, RBX-3, RBX-4, RBX-5, RBX-6, RBX-7, dan RBX-8 berhasil diisolasi dari jaringan spons Xestospongia sp. Hasil uji antimikroba menunjukkan bahwa dari kedelapan isolat bakteri asosiasi spons Xestospongia sp., tidak menunjukkan zona penghambatan terhadap bakteri uji Pasteurella multocida. Hasil identifikasi gen 16S rRNA menunjukkan keberhasilan isolasi DNA dan amplifikasi menggunakan PCR pada ukuran ~1500 pb. Hasil sekuensing menunjukkan bahwa isolat RBX 2 memiliki kemiripan dengan Micrococcus yunnanensis strain 190306H242 (99.77%), isolat RBX 4 menunjukan kemiripan dengan Kocuria rhizophila strain Ag09 (99.51%), dan RBX 8 menunjukan kemiripan dengan Oceanobacillus kimchii strain MXR1709B02 (99.28%). Kesimpulan penelitian ini adalah, bakteri asosiasi spons dapat diisolasi, penelitin ini berhasil membuktikan bahwa bakteri asosiasi Xestospongia sp. asal perairan sabang tidak memiliki aktivitas anti mikroba pada bakteri gram negative, dan isolat bakteri dapat diidentifikasi dengan 16S rRNA dan dapat di sekuensing. Kata kunci: 16S rRNA, bakteri asosiasi, endofit, spons, Xestospongia sp.

ABSTRACT Sponges produce bioactive compounds derived from association bacteria that have potential as antimicrobials. This study aims to isolate and identify the DNA of sponge-associated bacteria based on the analysis of the 16S rRNA gene. The results showed that eight isolates of sponge association bacteria RBX-1, RBX-2, RBX-3, RBX-4, RBX-5, RBX-6, RBX-7, and RBX-8 were isolated from the sponge tissue of Xestospongia sp. The antimicrobial test results showed that of the eight isolates of the sponge association bacteria Xestospongia sp., there was no zone of inhibition against the test bacteria Pasteurella multocida. The results of the identification of the 16S rRNA gene showed the success of DNA isolation and amplification using PCR at a size of ~1500 bp. Sequencing results showed that RBX 2 isolate had similarities with Micrococcus yunnanensis strain 190306H242 (99.77%), RBX 4 isolate showed similarities with Kocuria rhizophila strain Ag09 (99.51%), and RBX 8 showed similarity with Oceanobacillus kimchii strain MXR1709B02 (99.28%). The conclusion of this study is that sponge association bacteria can be isolated, this study succeeded in proving that Xestospongia sp. from Sabang waters did not have antimicrobial activity on gramnegative bacteria, and bacterial isolates could be identified by 16S rRNA and could be sequenced. Keywords: 16S rRNA, association bacteria, endophytes, sponge, Xestospongia sp.