Electronic Theses and Dissertation

ABSTRAK

Kajian cross-sectional pencemaran bakteri E s c h e r i c h i a coli pada tiga peternakan ayam petelur telah dilakukan pada bulan Februari 2021 di Dusun Silaok, Kecamatan Mungka, Kabupaten Limapuluh Kota, Provinsi Sumatera Barat. Penelitian ini bertujuan mengetahui tingkat pencemaran bakteri E.coli pada telur ayam ras petelur (Gallus sp.) dan faktor-faktor resiko yang mempengaruhi terjadinya pencemaran, yang berguna sebagai salah satu referensi dalam pencegahan pencemaran bakteri E.coli pada peternakan ayam petelur. Penentuan objek penelitian yaitu tiga peternakan ayam petelur dipilih berdasarkan metoda purpusive sampling. Sedangkan pengambilan sampel telur pada masing-masing peternakan terpilih dilakukan secara acak pada setiap unit sampling (ikatan tray telur). Pengamatan bakteri Escherichia coli dilakukan berdasarkan morfologi koloni Escherichia coli yang ditanam pada media Eosin Methylene Blue Agar (EMBA). Data hasil penelitian dianalisis menggunakan uji Fisher exact test dan uji Odds Ratio. Hasil pengamatan diketahui bahwa ke tiga peternakan ayam petelur yang diamati terjadi pencemaran bakteri Escherichia coli. Tingkat pencemaran telur oleh bakteri Escherichia coli mencapai 33% sampai 100% dengan jumlah rata-rata bakteri Escherichia coli 0,3x101 cfu/gram sampai 3,3x101cfu/gram. Hasil perhitungan Fisher Exact Test diperoleh bahwa faktor pendidikan sarjana dan keberadaan gudang penyimpan telur berpengaruh nyata dalam menurunkan tingkat pencemaran Escherichia coli pada telur di peternakan. Namun berdasarkan odds ratio diperoleh faktor-faktor yang berpengaruh adalah kebersihan kandang, waktu retensi telur, jumlah populasi ayam dan produksi telur.

Kata Kunci : Telur ayam ras, Escherichia coli, analisis odds ratio


ABSTRACT

A cross-sectional study of bacterial contamination of Escherichia coli on three layer chicken farms was conducted in February 2021 in Silaok Hamlet, Mungka District, Limapuluh Kota Regency, West Sumatra Province. This study aims to determine the level of contamination of E.coli bacteria in laying hens (Gallus sp.) eggs and the risk factors that influence the occurrence of pollution, which is useful as a reference in preventing E.coli bacterial contamination in laying hens farms. Determination of the object of research, namely three laying hens were selected based on the purpusive sampling method. Meanwhile, egg sampling on each selected farm was carried out randomly in each sampling unit (egg tray bond). Observation of Escherichia coli bacteria was carried out based on the morphology of Escherichia coli colonies grown on Eosin Methylene Blue Agar (EMBA) media. The research data were analyzed using the Fisher exact test and the Odds Ratio test. The results of the observations revealed that the three laying hens that were observed were contaminated with Escherichia coli bacteria. The level of egg contamination by Escherichia coli bacteria reaches 33% to 100% with an average number of Escherichia coli bacteria from 0.3x101 cfu/gram to 3.3x101 cfu/gram. The results of the Fisher Exact Test calculation showed that undergraduate education factors and the existence of egg storage warehouses had a significant effect in reducing the level of Escherichia coli contamination in eggs on farms. However, based on the odds ratio, the influencing factors are the cleanliness of the cage, egg retention time, the number of chicken populations and egg production.


Keywords : Chicken eggs, Escherichia coli, Odds ratio analysis

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