Electronic Theses and Dissertation

CEMARAN Salmonella sp PADA BAHAN PANGAN ASAL HEWAN (BPAH)
DI KAPAL YANG SANDAR DI PELABUHAN MALAHAYATI ACEH BESAR

ABSTRAK

Penelitian ini bertujuan untuk mengetahui ada tidaknya cemaran Salmonella sp. pada bahan pangan asal hewan dan jumlah cemaran Salmonella sp serta mengidentifikasi penyebab terjadinya cemaran Salmonella sp. pada di kapal yang sandar di Pelabuhan Malahayati Aceh Besar. Penelitian ini menggunakan rancangan cross sectional study dengan sampel seluruh kapal angkut barang yang sandar di Pelabuhan Malahayati Aceh Besar pada bulan Maret 2021. Sampel adalah bahan pangan asal hewan yang terdiri dari daging, ikan dan daging ayam mentah dan telah dimasak yang diambil dari 3 kapal angkut barang yang bersandar minimal 3 hari di Pelabuhan Malahayati Aceh Besar. Metode pengujian yang digunakan antara lain total plate count, uji biokimia meliputi uji fisiologis (uji motil), uji Metil Red, uji Voges Proskauer, uji TSIA, uji KIA, uji simmons citrate dan uji fermentasi karbohidrat (glukosa, laktosa, sukrosa). Data yang diperoleh dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa cemaran Salmonella sp ditemukan pada ikan mentah (100%) pada semua kapal angkut, dengan jumlah Salmonella sp masing-masing 3,0x102; 10x102; 1x101 CFU/g, cemaran pada daging ayam mentah terdapat pada dua kapal (66,67%) masing-masing sebanyak 5x103 dan 1x101 CFU/g dan daging sapi mentah pada satu kapal (33,33%) yaitu sebanyak 1,3x102 CFU/g. Salmonella sp tidak ditemukan pada daging ayam masak, ikan masak, dan daging sapi masak. Berdasarkan hasil penelitian dapat disimpulkan bahwa terdapat cemaran Salmonella sp. pada ikan, daging ayam dan daging sapi mentah di kapal yang sandar di Pelabuhan Malahayati Aceh Besar. Jumlah cemaran Salmonella sp. pada di kapal yang sandar di Pelabuhan Malahayati Aceh Besar tergolong paling tinggi dibandingkan SNI tahun 2008. Tingkat cemaran Salmonella sp yang paling tinggi ditemukan pada bahan pangan ikan mentah 100%, daging ayam mentah 66,67%, dan daging mentah 33,33%. Jadi dapat disimpulkan bahwa cemaran Salmonella sp. pada di kapal yang sandar di Pelabuhan Malahayati Aceh Besar disebabkan karena suhu penyimpanan bahan pangan yang tidak sesuai standar.
Kata kunci : Salmonella sp., Cemaran, Bahan Pangan, kapal pengangkut, Pelabuhan Malahayati.


















Salmonella sp CONTAMINATION ON ANIMAL ORIGIN FOODS
ON SHIPS THAT DOCK AT MALAHAYATI PORT, ACEH BESAR

ABSTRACT

This study aims to determine whether there is contamination of Salmonella sp. on foodstuffs of animal origin and the amount of Salmonella sp. contamination as well as identifying the cause of Salmonella sp. contamination on on ships docked at Malahayati Port, Aceh Besar. This research is an observational study. All goods transport ships docked at Malahayati Port, Aceh Besar in March 2021, samples of food of animal origin in the form of meat chicken meat, and fish in raw and processed/cooked form were taken from 3 goods transporting ships that leaned at least 3 days at Malahayati Port, Aceh Besar. The methods used are microbiological test (isolation), examination of the number of plates, biochemical tests including physiological testing (motile test), Methyl Red test, Voges Proskauer test, TSIA test, MCH test, Simmons citrate test and carbohydrate, lactose, sucrose fermentation test ). The data obtained in the research related to the types of food ingredients of animal origin were analyzed descriptively, the data on the amount of Salmonella sp. contamination before and after being processed using the t test. The results showed that Salmonella sp. was found in raw fish on all transport ships, with the number of Salmonella sp each being 3.0x102; 10x102; 1x101 CFU/g (100%), contamination in raw chicken meat was found in two vessels of 5x103 and 1x101 CFU/g (66.67%), and raw beef on the second vessel was 1.3x102 CFU/g (33.33%). There is no salmonella sp. contamination in cooked chicken meat, fish found, and cooked beef. Based on the results of the study, it can be said that there is contamination of Salmonella sp. on raw fish, raw chicken and raw beef on ships docked at Malahayati Port, Aceh Besar. Total contamination of Salmonella sp. on on ships docked at Malahayati Port, Aceh Besar, is the highest compared to SNI in year 2008. The highest level of Salmonella sp. contamination was found in 100% raw fish food, 66.67% raw chicken meat, and 33.33% raw meat. It can be concluded that salmonella sp. contamination from food on ships docked at Malahayati Port, Aceh Besar, due to food storage temperatures that are not up to standard.
Keywords : Salmonella sp. contamination, foodstuffs, Transport Ships, Malahayati Port.

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