Electronic Theses and Dissertation

ABSTRAK
Yoghurt merupakan produk susu fermentasi yang mengandung probiotik dari golongan
bakteri asam laktat (BAL), dapat menghazilkan enzim pencernaan dalam meningkatkan
ketersediaan nutrient untuk proses metabolisme tubuh. Penelitian ini bertujuan mengetahui sebaran
karbohidrat dan menghitung jumlah sel goblet pada usus halus mencit setelah penambahan filtrat
pandan wangi pada yoghurt. Penelitian menggunakan Rancangan Acak Lengkap (RAL). Hewan
coba yang digunakan mencit (Mus musculus) jantan berumur 3-4 bulan, berat 20-30 gram.
Perlakuan penelitian dibagi menjadi 6 kelompok perlakuan,yaitu P0 sebagai kontrol; P1 diberikan
0,5 ml filtrat pandan wangi 17,5%; P2 diberikan 0,5 ml filtrat pandan wangi 22,5%; P3 0,5 ml
yoghurt; P4 0,5 ml yoghurt filtrat pandan wangi 17,5%; dan P5 0,5 ml yoghurt filtrat pandan
wangi 22,5% yang diberikan terhadap semua kelompok selama 14 hari. Nekropsi dan pengambilan
organ usus dilakukan pada hari ke-15. Pengamatan sebaran karbohidrat dilakukan dengan
pewarnaan alcian blue (AB) pH 2,5 untuk karbohidrat asam dan periodic acid schiff (PAS) untuk
karbohidrat netral. Data hasil penelitian dianalisis menggunakan Analisis Of Varians dan jumlah
sel goblet dianalisis dengan uji Krushkal-Wallis. Hasil penelitian menunjukkan bahwa sebaran
karbohidrat asam dan netral ditemukan di seluruh permukaan usus dan yang paling banyak
ditemukan di sel goblet. Penambahan yoghurt pandan wangi dengan konsentrasi filtrat 17,5%
dapat meningkatkan jumlah sel goblet yang mengandung karbohidrat asam dan tidak menurunkan
jumlah sel goblet yang mengandung karbohidrat netral.
Kata kunci: duodenum, karbohidrat asam dan netral, mencit, pandan wangi, yoghurt

ABSTRACT
Yoghurt is a fermented milk product containing probiotics from the lactic acid bacteria
(LAB) group, which can produce digestive enzymes to increase the availability of nutrients for the
body?s metabolic processes. This study aims to determine the distribution of carbohydrates and
count the number of goblet cells in the small intestine of mice after the addition of pandan wangi
filtrate to yoghurt. The study used a completely randomized design (CRD). The experimental
animals used were male mice (Mus musculus) 3-4 months old, weighing 20-30 grams. The
research treatments were divided into six treatments, namely P0 as a control, P1 given 0.5 ml of
17.5% pandan filtrate, P2 0.5 ml of 22.5% pandan filtrate, P3 0.5 ml of yoghurt, P4 0.5 ml of
17.5% pandan wangi filtrate yoghurt, and 0.5 ml of 22.5% pandan wangi filtrate yoghurt which
was given for 14 days. Necropsy and intestinal organ harvesting were performed on the 15th day.
Observation of carbohydrate distribution was carried out by staining Alcian blue (AB) pH 2.5 for
acidic carbohydrates and periodic acid schiff (PAS) for neutral carbohydrates. The research data
were analyzed using Analysis Of Variance and the number of goblet cells was analyzed using the
Krushkal-Wallis test. The results showed that the distribution of acidic and neutral carbohydrates
was found on the entire surface of the intestine and the most abundant was found in goblet cells.
The addition of pandan wangi yoghurt with a filtrate concentration of 17.5% can increase the
number of goblet cells containing acidic carbohydrates and not reduce the number of goblet cells
containing neutral carbohydrates.
Keywords: duodenum, acidic and neutral carbohydrate, mice, pandan wangi, yoghur

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