Electronic Theses and Dissertation

ABSTRAK

Yoghurt merupakan minuman kesehatan yang dihasilkan melalui proses fermentasi oleh bakteri asam laktat (BAL) yang bermanfaat dalam meningkatkan kekebalan tubuh. Yoghurt dapat bersifat sebagai imunostimulan yang ditandai dengan peningkatan jumlah sel imun dan peningkatan diameter organ yang memproduksi sel imun. Penelitian ini bertujuan untuk mengetahui diameter pulpa putih limpa dan jumlah limfosit darah mencit setelah pemberian yoghurt yang ditambahkan filtrat pandan wangi. Hewan coba yang digunakan mencit (Mus musculus) jantan umur 3-4 bulan, berat 20-30 gram. Perlakuan penelitian dibagi menjadi enam kelompok perlakuan, P0 sebagai kontrol, P1 diberikan 0,5 ml yoghurt, P2 0,5 ml filtrat pandan wangi 17,5%, P3 0,5 ml filtrat pandan wangi 22,5%, P4 0,5 ml yoghurt filtrat pandan wangi 17,5%, dan P5 0,5 ml yoghurt filtrat pandan wangi 22,5% yang diberikan selama 14 hari. Pengambilan sampel darah dan limpa dilakukan pada hari ke-15. Penghitungan diameter pulpa putih menggunkan metode parafin dan pewarnaan Hematoxylin Eosin (HE) dan jumlah limfosit dihitung menggunakan metode apus darah. Data hasil penelitian dianalisis menggunakan one way analisis of varians (ANOVA) dan dilanjutkan dengan uji Duncan. Hasil analisis statistik menunjukan bahwa terjadi peningkatan diameter pulpa putih limpa antar kelompok perlakuan, diameter pulpa putih tertinggi pada kelompok P5 yaitu sebesar 424.09 ± 15,75 µm dan tidak terjadi peningkatan jumlah limfosit darah pada masing-masing kelompok perlakuan. Berdasarkan hasil penelitian dapat disimpulkan bahwa pemberian yoghurt filtrat pandan wangi meningkatkan diameter pulpa putih limpa dan tidak meningkatkan jumlah limfosit darah pada mencit (Mus musculus).



Kata kunci: yoghurt pandan wangi, limfosit, diameter pulpa putih, mencit


ABSTRACT

Yoghurt is a health drink produced through a fermentation process by lactic acid bacteria (LAB) which is beneficial in increasing immunity. Yogurt can act as an immunostimulant by increasing the number of immune cells and the diameter of organ that produces immune cells. The purpose of this study is aimed to determine the diameter of white pulp and the number of mice’s blood lymphocytes after giving yoghurt with pandan wangi filtrate. The laboratory animals that used were male mice (Mus musculus) 3-4 months, weighing 20-30 grams. The research divided into six groups of treatments, P0 as a control, P1 was given 0.5 ml of yoghurt, P2 0.5 ml of pandan filtrate 17.5%, P3 0.5 ml of pandan filtrate 22.5%, P4 0.5 ml yoghurt pandan wangi filtrate 17,5%, and P5 0.5 ml of pandan wangi filtrate 22,5% which were given for 14 days. Blood and spleen samples were taken on the 15th day. The white pulp diameter on spleen was calculated using the paraffin method and Hematoxylin Eosin (HE) staining and the number of lymphocyte was calculated by blood smear method. Data were analyzed with one way analysis of variance (ANOVA) and continued with Duncan's test. The results of statistical analysis showed that there was an increase in the diameter of white pulp on spleen between groups of treatment, the highest of white pulp diameter in the P5 group was 424.09 ± 15.75 µm and there was no enhancement on the number of blood lymphocytes in each treatment group. Based on the results of this study, it can be concluded that the giving of yoghurt with pandan wangi filtrate increased the diameter of the white pulp’s spleen and did not increase the number of blood lymphocytes in mice (Mus musculus).


Keywords: yoghurt, pandan wangi, lymphocytes, diameter of the spleen white pulp, mice

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