Electronic Theses and Dissertation

ABSTRAK

Diferensiasi sel spermatid menjadi spermatozoa dapat diidentifikasi berdasarkan proses pembentukan akrosom pada sel spermatid di dalam tubulus seminiferus testis. Penelitian ini bertujuan untuk mengidentifikasi perkembangan sel spermatid pada setiap tahapan (stage) epitel seminiferus testis sapi aceh yang dideteksi dengan pewarnaan histokimia periodic acid Schiff (PAS). Penelitian ini menggunakan lima pasang testes yang diperoleh dari lima ekor sapi aceh jantan dewasa berumur 2-3 tahun. Organ testis dipisahkan dari skrotum dan tunica vaginalis parietalis et visceralis lalu difiksasi dalam larutan neutral buffer formalin (NBF 10%) dan diproses menjadi preparat histologi. Preparat testis diwarnai dengan teknik histokimia PAS dan data yang diperoleh dianalisis secara deskriptif. Hasil penelitian ini adalah terdapat delapan langkah (steps) perkembangan (diferensiasi) sel-sel spermatid mulai dari round spermatid sampai elongated spermatid yang diamati pada delapan tahap (stages) epitel tubulus seminiferus testis sapi aceh. Delapan langkah tersebut berada pada Golgi phase (langkah 1, 2, dan 3), cap phase (langkah 4 dan 5), acrosomal phase (langkah 6 dan 7), dan maturation phase (langkah 8). Kesimpulan penelitian ini adalah diferensiasi sel spermatid menjadi spermatozoa pada jaringan testis sapi aceh berlangsung dalam delapan langkah (steps) yang diamati dengan pewarnaan histokimia periodic acid Schiff (PAS).

Kata kunci: testis, spermatid, akrosom, karbohidrat netral, dan sapi aceh.

ABSTRACT

The differentiation of spermatid cells into spermatozoa can be identified based on the process of acrosome formation in the spermatid of the testicular seminiferous tubules. This study aimed to identify the development of spermatid cells in the seminiferous epithelial stage in the testicular tissue of the Aceh cattle detected by histochemical Schiff periodic acid (PAS) staining. This study used five pairs testes obtained from five adult Aceh cattle aged 2-3 years. The testicular organs were separated from the scrotum and tunica vaginalis parietalis et visceralis and then fixed in neutral buffer formalin (NBF 10%) and processed into histological preparations. Testicular preparations were stained with the PAS histochemical technique and the data obtained were analyzed descriptively. The results of this study showed that there were eight steps in the development (differentiation) of spermatids, from round to elongated spermatids which were observed at the eight stages (stages) of the testicular seminiferous tubular epithelium. The eight steps were in the Golgi phase (steps 1, 2, and 3), the cap phase (steps 4 and 5), the acrosomal phase (steps 6 and 7), and the maturation phase (step 8). The conclusion of this study the differentiated of spermatid cells into spermatozoa in the testicular tissue of Aceh cattle occurs in eight steps which observed used Schiff periodic acid (PAS) histochemical staining.

Keywords: testis, spermatid cells, acrosome, neutral carbohydrates, Aceh cattle

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