Electronic Theses and Dissertation

ABSTRAK

Daun Bandotan (Ageratum conyzoides) merupakan tanaman yang diduga memiliki sifat antibakteri. Penelitian ini bertujuan mengetahui sensitivitas ekstrak daun Bandotan dalam menghambat pertumbuhan bakteri Pseudomonas aeruginosa. Penelitian ini menggunakan stok ekstrak daun Bandotan dari Laboratorium Farmakologi dan isolat bakteri Pseudomonas aeruginosa di Laboratorium Mikrobiologi FKH USK yang direidentifikasi dengan pewarnaan Gram, uji indol, uji Methyl Red, dan uji gula-gula. Metode penelitian dilakukan dengan penanaman isolat bakteri yang telah direidentifikasi pada media Nutrient Broth (NB), diinkubasi pada suhu 37ºC selama 24 jam. Kemudian disusun komposisi kekeruhan isolat hingga sama dengan kekeruhan pada larutan 0,5 McFarland. Selanjutnya uji sensitivitas ekstrak pada media Mueller Hinton Agar (MHA) dilakukan dengan meratakan isolat bakteri pada permukaan media dan ditempelkan disk yang berisi ekstrak daun bandotan dengan konsentrasi 25%, 50%, 75% dan disk gentamisin sebagai kontrol positif serta aquades sebagai kontrol negatif, semua perlakuan diinkubasi pada suhu 37ºC selama 24 jam, kemudian dilakukan pengukuran zona hambat menggunakan jangka sorong dengan satuan milimeter (mm). Hasil penelitian menunjukkan bahwa pada konsentrasi 25%, 50% dan 75% masing-masing memiliki zona hambat ber-urutan sebesar 8,16 mm, 9,82 mm dan 16,08 mm, sedangkan pada antibiotik gentamisin rata-rata zona hambat 25,30 mm dan aquades 0 mm. Dapat disimpulkan bahwa ekstrak daun Bandotan memiliki sensitivitas terhadap hambatan pertumbuhan bakteri Pseudomonas aeruginosa.

Kata kunci: Daun Bandotan, Ageratum conyzoides, Pseudomonas aeruginosa, antibakteri
ABSTRAC

The leaves of Bandotan (Ageratum conyzoides) are a plant thought to have antibacterial properties. This study aims to determine the sensitivity of Bandotan leaf extract in inhibiting the growth of the bacteria Pseudomonas aeruginosa. This study used a stock extract of Bandotan leaves from the Pharmacology Laboratory and a bacterial isolate of Pseudomonas aeruginosa in the Microbiology Laboratory of the USK FKH which was identified by Gram staining, indole test, Methyl Red test, and confectionery test. The research method was carried out by planting the re-identified bacterial isolates on Nutrient Broth (NB) media, incubated at 37ºC for 24 hours. Then the turbidity composition of the isolates was arranged to match the turbidity in 0.5 McFarland solution. Furthermore, the sensitivity test of the extract on Mueller Hinton Agar (MHA) media was carried out by leveling the bacterial isolates on the surface of the media and attaching a disc containing bandotan leaf extract with a concentration of 25%, 50%, 75% and gentamicin disk as a positive control and distilled water as a negative control. All treatments were incubated at 37ºC for 24 hours, then the inhibition zone was measured using a calipers in millimeters (mm). The results showed that at concentrations of 25%, 50% and 75%, respectively, had an inhibition zone of 8.16 mm, 9.82 mm and 16.08 mm respectively, whereas the average inhibition zone for gentamicin was 25, 30 mm and 0 mm distilled water. It can be concluded that the Bandotan leaf extract has sensitivity to growth inhibition of Pseudomonas aeruginosa bacteria.

Keywords: Bandotan leaves, Ageratum conyzoides, Pseudomonas aeruginosa, antibacterial

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