Electronic Theses and Dissertation

ABSTRAK
Uji aktivitas antibakteri ekstrak metanol daun gaharu (Aquilaria malaccensis) terhadap
pertumbuhan bakteri Staphylococcus aureus dan Escherichia coli telah dilakukan
untuk mengetahui aktivitas ekstrak metanol daun gaharu dalam menghambat
pertumbuhan bakteri tersebut. Ekstrak dari sampel diperoleh melalui metode maserasi
dengan pelarut metanol. Simplisia daun gaharu mengandung kadar air sebesar 7,83%,
kadar abu total 5,64%, kadar sari larut air 17,5%, dan kadar sari larut etanol 29,17%.
Ekstrak metanol daun gaharu mengandung kadar air sebesar 12,83%, kadar abu total
4,77%, kadar sari larut air 28,33%, dan kadar sari larut etanol 44,17%. Hasil uji
fitokimia menunjukkan bahwa ekstrak mengandung senyawa alkaloid, saponin, tanin,
steroid, dan glikosida. Uji aktivitas antibakteri ekstrak metanol sampel dilakukan
dengan menggunakan metode sumuran dengan variasi konsentrasi ekstrak yaitu 5; 10;
15; 20 dan 25%. Aktivitas antibakteri terhadap S. aureus pada ekstrak metanol daun
gaharu lebih tinggi dibandingkan dengan aktivitas ekstrak metanol pada bakteri E. coli.
Aktivitas tertinggi ekstrak metanol daun gaharu terhadap S. aureus dan E. coli
diperoleh pada konsentrasi 25% dengan zona hambat masing-masing 12 mm dan 7,8
mm.
Kata kunci: Daun Gaharu, Aquilaria malaccensis, Ekstrak metanol, antibakteri

ABSTRACT
Antibacterial activity test of methanolic extract from agarwood leaves (Aquilaria
malaccensis) towards bacterial growth of Staphylococcus aureus and Escherichia
coli has been conducted to know the activity of metanolic extract from agarwood
leaves in inhibiting the growth of those bacterials. The extract of the samples were
obtained through maceration method by methanol as a solvent. Agarwood leaves
simplicial characterization contains water content of 7,83%, total ash content of
5,64%, water soluble extract content of 17,5%, and ethanol soluble extract content of
29,17%. Methanolic extract from agarwood leaves contains water content of
2812,83%, total ash content of 4,77%, water soluble extract content of 28,33%, and
ethanol soluble extract content of 44,17%. Phytochemical test showed that both of
extract contained alkaloid compounds, saponins, tannins, steroids, and glycosides.
Antibacterial activity test of methanolic extract from samples was conducted using a
well diffusion method with variation of extract concentrations of 5; 10; 15; 20 and
25%. Antibacterial activity towards bacterial of S. aureus at methanolic extract from
agarwood leaves is higher than activity of methanol extract towards bacterial of E.
coli. The highest activity of methanolic extract from agarwood leaves towards S.
aureus and E.coli were obtained at 25% concentration with inhibiting zone diameter
of 12 mm and 7,8 mm.
Keywords: Agarwood leaves, Aquilaria malaccensis, methanolic extract,
antibacterial.

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