Electronic Theses and Dissertation

STRACT
Penelitian ini bertujuan untuk menganalisis pengaruh pemberian ekstrak etanol kulit
batang Cyathocalyx sumatranus (EEKBCS) terhadap kadar serum glutamat
oxaloasetat transaminase (SGOT) dan serum glutamat piruvat transaminase (SGPT)
tikus yang diinduksi parasetamol dosis toksik, serta mengetahui konsentrasi
antioksidan ekstrak etanol C. sumatranus. Metode yang digunakan dalam penelitian
ini adalah Rancangan Acak Lengkap (RAL) yang terdiri dari 6 kelompok perlakuan
dan 4 ulangan. Hewan uji yang digunakan ialah tikus jantan galur Wistar usia 3
bulan dengan rerata berat badan 271,375± 15,251 g sebanyak 24 individu diinduksi
hepatotoksisitas dengan parasetamol sebanyak 1350 mg/kgBB pada hari ke-7 kecuali
kontrol normal (K0), yang diberi akuades. Hewan uji juga mendapat perlakuan
pemberian akuades pada kontrol negatif (K-), Hepa-Q sebanyak 11,34 mg/kgBB
kontrol positif (K+), EEKBCS dosis 150 mg/kgBB (P1), 300 mg/kgBB (P2) dan 600
mg/kgBB (P3) selama 14 hari. Data dianalisis menggunakan Analysis of Variance
(ANOVA) taraf signifikan 5% dengan uji Tukey sebagai uji lanjut. Hasil penelitian
menunjukkan bahwa terdapat pengaruh pemberian EEKBCS terhadap kadar SGOT
dan SGPT. Berdasarkan hasil penelitian disimpulkan bahwa dosis 300 mg/kgBB
EEKBCS memiliki potensi hepatoprotektor. Hasil pengukuran secara
spektrofotometri menunjukkan bahwa EEKBCS memiliki nilai IC50 sebesar 94,906
?g/mL sehingga aktivitas antioksidan EEKBCS dikategorikan sebagai aktivitas
antioksidan kuat.
Kata Kunci : SGOT, SGPT, C. sumatranus, hepatoprotektor, antioksidan.
ABSTRACT
This study aims to analyze the effect of ethanol extract of stem Cyathocalyx
sumatranus bark (EEKBCS) on serum glutamate oxaloacetate transaminase (SGOT)
and serum glutamate pyruvate transaminase (SGPT) rats induced by toxic doses of
paracetamol, and to determine the antioxidant concentration of ethanol extract C.
sumatranus. The method used in this study was a completely randomized design
(CRD) consisting of 6 treatment groups and 4 replications. The test animals used
were male Wistar rats aged 3 months with a mean body weight of 271.375 ± 15.251
g, as many as 24 individuals were induced hepatotoxicity with 1350 mg/kgBW of
paracetamol on the 7th day except for normal control (K0), which were given
distilled water. Test animals were also treated with distilled water to negative control
(K-), Hepa-Q as much as 11.34 mg/kgBW positive control (K +), EEKBCS dose of
150 mg / kgBW (P1), 300 mg/kgBW (P2) and 600 mg/kgBW (P3) for 14 days. Data
were analyzed using Analysis of Variance (ANOVA) with a significant level of 5%
with Tukey's test as a follow-up test. The results showed that there was an effect of
EEKBCS on SGOT and SGPT levels. Based on the results of the study, it was
concluded that the dose of 300 mg/kgBW EEKBCS has a potential hepatoprotector.
The results of spectrophotometric measurements showed that EEKBCS had an IC50

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