Rabiatul Adawiyah Primary Author mixed material bibliography Banda Aceh Universitas Syiah Kuala 2021 id Indonesia

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STRACT Penelitian ini bertujuan untuk menganalisis pengaruh pemberian ekstrak etanol kulit batang Cyathocalyx sumatranus (EEKBCS) terhadap kadar serum glutamat oxaloasetat transaminase (SGOT) dan serum glutamat piruvat transaminase (SGPT) tikus yang diinduksi parasetamol dosis toksik, serta mengetahui konsentrasi antioksidan ekstrak etanol C. sumatranus. Metode yang digunakan dalam penelitian ini adalah Rancangan Acak Lengkap (RAL) yang terdiri dari 6 kelompok perlakuan dan 4 ulangan. Hewan uji yang digunakan ialah tikus jantan galur Wistar usia 3 bulan dengan rerata berat badan 271,375± 15,251 g sebanyak 24 individu diinduksi hepatotoksisitas dengan parasetamol sebanyak 1350 mg/kgBB pada hari ke-7 kecuali kontrol normal (K0), yang diberi akuades. Hewan uji juga mendapat perlakuan pemberian akuades pada kontrol negatif (K-), Hepa-Q sebanyak 11,34 mg/kgBB kontrol positif (K+), EEKBCS dosis 150 mg/kgBB (P1), 300 mg/kgBB (P2) dan 600 mg/kgBB (P3) selama 14 hari. Data dianalisis menggunakan Analysis of Variance (ANOVA) taraf signifikan 5% dengan uji Tukey sebagai uji lanjut. Hasil penelitian menunjukkan bahwa terdapat pengaruh pemberian EEKBCS terhadap kadar SGOT dan SGPT. Berdasarkan hasil penelitian disimpulkan bahwa dosis 300 mg/kgBB EEKBCS memiliki potensi hepatoprotektor. Hasil pengukuran secara spektrofotometri menunjukkan bahwa EEKBCS memiliki nilai IC50 sebesar 94,906 ?g/mL sehingga aktivitas antioksidan EEKBCS dikategorikan sebagai aktivitas antioksidan kuat. Kata Kunci : SGOT, SGPT, C. sumatranus, hepatoprotektor, antioksidan. ABSTRACT This study aims to analyze the effect of ethanol extract of stem Cyathocalyx sumatranus bark (EEKBCS) on serum glutamate oxaloacetate transaminase (SGOT) and serum glutamate pyruvate transaminase (SGPT) rats induced by toxic doses of paracetamol, and to determine the antioxidant concentration of ethanol extract C. sumatranus. The method used in this study was a completely randomized design (CRD) consisting of 6 treatment groups and 4 replications. The test animals used were male Wistar rats aged 3 months with a mean body weight of 271.375 ± 15.251 g, as many as 24 individuals were induced hepatotoxicity with 1350 mg/kgBW of paracetamol on the 7th day except for normal control (K0), which were given distilled water. Test animals were also treated with distilled water to negative control (K-), Hepa-Q as much as 11.34 mg/kgBW positive control (K +), EEKBCS dose of 150 mg / kgBW (P1), 300 mg/kgBW (P2) and 600 mg/kgBW (P3) for 14 days. Data were analyzed using Analysis of Variance (ANOVA) with a significant level of 5% with Tukey's test as a follow-up test. The results showed that there was an effect of EEKBCS on SGOT and SGPT levels. Based on the results of the study, it was concluded that the dose of 300 mg/kgBW EEKBCS has a potential hepatoprotector. The results of spectrophotometric measurements showed that EEKBCS had an IC50 0 ELECTRONIC THESES AND DISSERTATION Universitas Syiah Kuala 87420 2021-01-14 17:31:05 2021-01-15 08:43:26 machine generated