Electronic Theses and Dissertation

Abstrak. Penelitian ini bertujuan untuk mengetahui pengaruh penggunaan rizobakteri
sebagai agens biokontrol dan pemacu pertumbuhan tanaman untuk induksi ketahanan
secara sistemik terhadap patogen JAP pada tanaman pala fase bibit. Penelitian
dilaksanakan di Laboratorium Ilmu dan Teknologi Benih Jurusan Agroteknologi Fakultas
Pertanian Universitas Syiah Kuala, Darussalam Banda Aceh. Penelitian ini berlangsung
pada bulan Februari sampai Agustus 2020. Rancangan yang digunakan pada penelitian ini
adalah Rancangan Acak Lengkap (RAL) Non Faktorial. Faktor yang diteliti adalah perbedaan
isolat rizobakteri dalam menghambat JAP yang terdiri atas 18 jenis isolat rizobakteri, yaitu :
AP 3/1 (R1), AP 3/10 (R2), AP 3/11 (R3), AP 4/1 (R4), AP 4/5 (R5), AP 5/3 (R6), AP 5/5 (R7),
AP 5/6 (R8), AP 5/7 (R9), AP 5/8 (R10), AP 6/1 (R11), AP 7/1 (R12), AP 7/2 (R13), AP 7/3
(R14), AP 7/4 (R15), AP 7/5 (R16), AP 7/6 (R17), AP 8/2 (R18), dan (R0) sebagai kontrol.
Setiap taraf percobaan diulang sebanyak 3 kali dan masing masing ulangan ditanam
dengan 10 tanaman. Parameter yang diamati adalah tinggi batang, diameter pangkal
batang, jumlah daun, aktivitas enzim peroksidase, dan persentase kejadian penyakit. Hasil
penelitian menunjukkan bahwa pertumbuhan tinggi bibit pala umur 145 HST dan 159 HST
yang cenderung tertinggi dijumpai pada perlakuan rizobakteri AP 5/3, diameter bibit pala
umur 145 HST dan 159 HST yang cenderung tertinggi dijumpai pada perlakuan rizobakteri
AP 3/1, jumlah daun bibit pala umur 145 HST cenderung lebih besar dijumpai pada
perlakuan rizobakteri AP 7/6, dan pada umur 159 HST jumlah daun bibit pala cenderung
lebih besar dijumpai pada perlakuan rizobakteri AP 5/6. Hasil penelitian juga menunjukkan
bahwa Dari hasil uji aktivitas enzim peroksidase, didapatkan aktivitas tertinggi pada bibit
pala yang diberi perlakuan isolat rizobakteri AP 3/1, yaitu 1,3067 U mg-1
protein. Dari
perhitungan persentase kejadian penyakit pada bibit pala yang telah di inokulasi oleh
patogen JAP, di dapat bibit pala yang diberi perlakuan isolat rizobakteri AP 7/5 mampu
bertahan dari serangan koloni patogen JAP dengan persentase kejadian penyakit hanya
1,28%.
Kata kunci : Agens Biokontrol, Inokulasi, Peroksidase, Rizobakteri
Abstract. This study aims to determine the effect of using rhizobacteria as biocontrol
agents and plant growth promoters to induce systemic resistance to JAP pathogens in
seedling phase nutmeg plants. The research was conducted at the Laboratory of Seed
Science and Technology, Department of Agrotechnology, Faculty of Agriculture, Syiah
Kuala University, Darussalam Banda Aceh. This research took place from February to
August 2020. The design used in this study was a Non-factorial Completely Randomized
Design (CRD). The factors studied were differences in rhizobacterial isolates in inhibiting
JAP which consisted of 19 treatments, namely: Control ((R0), AP 3/1 (R1), AP 3/10 (R2),
AP 3/11 (R3), AP 4/1 (R4), AP 4/5 (R5), AP 5/3 (R6), AP 5/5 (R7), AP 5/6 (R8), AP 5 / 7
(R9), AP 5/8 (R10), AP 6/1 (R11), AP 7/1 (R12), AP 7/2 (R13), AP 7/3 (R14) , AP 7/4 (R15),
AP 7/5 (R16), AP 7/6 (R17), and AP 8/2 (R18). Each stage of the experiment was repeated 3
times and each replication was planted with 10 plants. The parameters observed were stem
height, stem base diameter, number of leaves, peroxidase enzyme activity, and percentage
of disease incidence The results showed that the highest growth of nutmeg seedlings aged
145 DAS and 159 DAS was found in the rhizobacterial treatment of AP 5 / 3, the diameter
of nutmeg seedlings aged 145 DAS and 159 DAS tended to be the highest in the treatment
of rhizobacteria AP 3/1, the number of leaves of nutmeg seedlings aged 145 DAS tended to
be higher in It was found in AP 7/6 rhizobacteria treatment, and at the age of 159 DAS the
number of leaves of nutmeg seeds tended to be greater in the treatment of AP 5/6
rhizobacteria. The results also showed that from the results of the peroxidase enzyme
activity test, the highest activity was obtained in the nutmeg seeds treated with AP 3/1
rhizobacterial isolates, namely 1.3067 U mg
-1
protein. From the calculation of the
percentage of disease incidence in the nutmeg seeds that have been inoculated by the JAP
pathogen, it was found that the nutmeg seeds treated with the rhizobacterial isolate AP 7/5
were able to withstand the attack of the JAP pathogenic colony with a disease incidence
percentage of only 1.28%.
Key words: Biocontrol Agents, Inoculation, Peroxidase, Rhizobacteria

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