Ulfa Mahera Primary Author mixed material bibliography Banda Aceh Universitas Syiah Kuala 2020 id Indonesia

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Abstrak. Penelitian ini bertujuan untuk mengetahui pengaruh penggunaan rizobakteri sebagai agens biokontrol dan pemacu pertumbuhan tanaman untuk induksi ketahanan secara sistemik terhadap patogen JAP pada tanaman pala fase bibit. Penelitian dilaksanakan di Laboratorium Ilmu dan Teknologi Benih Jurusan Agroteknologi Fakultas Pertanian Universitas Syiah Kuala, Darussalam Banda Aceh. Penelitian ini berlangsung pada bulan Februari sampai Agustus 2020. Rancangan yang digunakan pada penelitian ini adalah Rancangan Acak Lengkap (RAL) Non Faktorial. Faktor yang diteliti adalah perbedaan isolat rizobakteri dalam menghambat JAP yang terdiri atas 18 jenis isolat rizobakteri, yaitu : AP 3/1 (R1), AP 3/10 (R2), AP 3/11 (R3), AP 4/1 (R4), AP 4/5 (R5), AP 5/3 (R6), AP 5/5 (R7), AP 5/6 (R8), AP 5/7 (R9), AP 5/8 (R10), AP 6/1 (R11), AP 7/1 (R12), AP 7/2 (R13), AP 7/3 (R14), AP 7/4 (R15), AP 7/5 (R16), AP 7/6 (R17), AP 8/2 (R18), dan (R0) sebagai kontrol. Setiap taraf percobaan diulang sebanyak 3 kali dan masing masing ulangan ditanam dengan 10 tanaman. Parameter yang diamati adalah tinggi batang, diameter pangkal batang, jumlah daun, aktivitas enzim peroksidase, dan persentase kejadian penyakit. Hasil penelitian menunjukkan bahwa pertumbuhan tinggi bibit pala umur 145 HST dan 159 HST yang cenderung tertinggi dijumpai pada perlakuan rizobakteri AP 5/3, diameter bibit pala umur 145 HST dan 159 HST yang cenderung tertinggi dijumpai pada perlakuan rizobakteri AP 3/1, jumlah daun bibit pala umur 145 HST cenderung lebih besar dijumpai pada perlakuan rizobakteri AP 7/6, dan pada umur 159 HST jumlah daun bibit pala cenderung lebih besar dijumpai pada perlakuan rizobakteri AP 5/6. Hasil penelitian juga menunjukkan bahwa Dari hasil uji aktivitas enzim peroksidase, didapatkan aktivitas tertinggi pada bibit pala yang diberi perlakuan isolat rizobakteri AP 3/1, yaitu 1,3067 U mg-1 protein. Dari perhitungan persentase kejadian penyakit pada bibit pala yang telah di inokulasi oleh patogen JAP, di dapat bibit pala yang diberi perlakuan isolat rizobakteri AP 7/5 mampu bertahan dari serangan koloni patogen JAP dengan persentase kejadian penyakit hanya 1,28%. Kata kunci : Agens Biokontrol, Inokulasi, Peroksidase, Rizobakteri Abstract. This study aims to determine the effect of using rhizobacteria as biocontrol agents and plant growth promoters to induce systemic resistance to JAP pathogens in seedling phase nutmeg plants. The research was conducted at the Laboratory of Seed Science and Technology, Department of Agrotechnology, Faculty of Agriculture, Syiah Kuala University, Darussalam Banda Aceh. This research took place from February to August 2020. The design used in this study was a Non-factorial Completely Randomized Design (CRD). The factors studied were differences in rhizobacterial isolates in inhibiting JAP which consisted of 19 treatments, namely: Control ((R0), AP 3/1 (R1), AP 3/10 (R2), AP 3/11 (R3), AP 4/1 (R4), AP 4/5 (R5), AP 5/3 (R6), AP 5/5 (R7), AP 5/6 (R8), AP 5 / 7 (R9), AP 5/8 (R10), AP 6/1 (R11), AP 7/1 (R12), AP 7/2 (R13), AP 7/3 (R14) , AP 7/4 (R15), AP 7/5 (R16), AP 7/6 (R17), and AP 8/2 (R18). Each stage of the experiment was repeated 3 times and each replication was planted with 10 plants. The parameters observed were stem height, stem base diameter, number of leaves, peroxidase enzyme activity, and percentage of disease incidence The results showed that the highest growth of nutmeg seedlings aged 145 DAS and 159 DAS was found in the rhizobacterial treatment of AP 5 / 3, the diameter of nutmeg seedlings aged 145 DAS and 159 DAS tended to be the highest in the treatment of rhizobacteria AP 3/1, the number of leaves of nutmeg seedlings aged 145 DAS tended to be higher in It was found in AP 7/6 rhizobacteria treatment, and at the age of 159 DAS the number of leaves of nutmeg seeds tended to be greater in the treatment of AP 5/6 rhizobacteria. The results also showed that from the results of the peroxidase enzyme activity test, the highest activity was obtained in the nutmeg seeds treated with AP 3/1 rhizobacterial isolates, namely 1.3067 U mg -1 protein. From the calculation of the percentage of disease incidence in the nutmeg seeds that have been inoculated by the JAP pathogen, it was found that the nutmeg seeds treated with the rhizobacterial isolate AP 7/5 were able to withstand the attack of the JAP pathogenic colony with a disease incidence percentage of only 1.28%. Key words: Biocontrol Agents, Inoculation, Peroxidase, Rhizobacteria 0 ELECTRONIC THESES AND DISSERTATION Universitas Syiah Kuala 80736 2020-10-05 11:20:38 2020-10-05 14:12:40 machine generated