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  <title>PERSIAPAN, PELAKSANAAN NEKROPSI DAN KOLEKS SAMPEL HISTOPATOLOGI PADA KASUSNEWCASTLE DISEASE</title>
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  <namePart>PUTRI AYU LESTARI</namePart>
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   <placeTerm type="text">Banda Aceh</placeTerm>
   <publisher>Universitas Syiah Kuala</publisher>
   <dateIssued>2020</dateIssued>
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 <note>PERSIAPAN, PELAKSANAAN NEKROPSI DAN KOLEKSI SAMPEL&#13;
HISTOPATOLOGI PADA KASUS NEWCASTLE DISEASE &#13;
&#13;
Putri Ayu Lestari (1702001010024) &#13;
&#13;
Dr. drh. Etriwati M,Si &#13;
 &#13;
 &#13;
ABSTRAK &#13;
 Nekropsi atau bedah bangkai hewan bertujuan untuk menetapkan diagnosa&#13;
penyakit atau penyebab kematian dari seekor hewan. Penulisan Tugas Akhir&#13;
tentang kasus Newcastle disease ini bertujuan untuk mengetahui bagaimana&#13;
persiapan sebelum nekropsi, pelaksanaan nekropsi yang benar dan cara koleksi&#13;
sampel untuk uji histopatologi. Nekropsi dilaksanakan di Laboratorium Patologi,&#13;
Unit Pelaksana Teknis Daerah Laboratorium Veteriner Dinas Peternakan dan&#13;
Kesehatan Hewan Aceh terhadap 2 ekor ayam broiler dan 1 ekor ayam kampung.&#13;
Sebelum dilakukan nekropsi dilakukan persiapan alat dan bahan untuk nekropsi&#13;
dan koleksi sampel. Pelaksanaan nekropsi diawali dengan pemeriksaan&#13;
sinyalemen, anamnesa dan keadaan luar bangkai, selanjutnya dilakukan nekropsi&#13;
dan diamati dan dicatat perubahan pada organ dan terakhir dilakukan koleksi&#13;
organ trakhea, paru-paru, duodenum, proventrikulus, seka tonsil dan otak. Dokter&#13;
Hewan menetapkan diagnosis Newcastle disease pada semua unggas yang di&#13;
nekropsi berdasarkan adanya lesi hemoragi/hipermi pada organ trakhea,&#13;
duodenum, proventrikulus, seka tonsil dan otak.  Fiksasi organ untuk uji&#13;
histopatologi menggunakan Netral Buffer Formalin (NBF 10%). Keterampilan&#13;
dan pengetahuan paramedik veteriner tentang persiapan alat yang dibutuhkan,&#13;
pelaksanaan nekropsi yang sesuai prosedur dan koleksi sampel berupa organ&#13;
trakhea, paru-paru, proventrikulus, usus dan otak dalan larutan NBF 10% sangat&#13;
mendukung dalam menentukan diagnosis Newcastle disease pada kasus lapang. &#13;
&#13;
Kata Kunci : Nekropsi, Newcastle disease, Koleksi sampel histopatologi &#13;
 &#13;
PREPARATION, IMPLEMENTATION OF NECROPSI AND&#13;
HISTOPATOLOGY SAMPLE COLLECTION IN NEWCASTLE             &#13;
DISEASE CASE&#13;
 &#13;
Putri Ayu Lestari (1702001010024)&#13;
 &#13;
Dr. drh. Etriwati, M.Si&#13;
&#13;
 &#13;
ABSTRACT &#13;
 Animal necropsy or surgery for the diagnosis of diseases or causes of&#13;
death from animals. The writing of the Final Project on the problem of Newcastle&#13;
disease is intended to find out how to prepare before necropsy, the correct&#13;
implementation of necropsy and how to collect samples for histopathological&#13;
testing. Necropsy was carried out at the Pathology Laboratory, Regional&#13;
Technical Implementation Unit of the Veterinary Laboratory of the Animal&#13;
Husbandry and Animal Health Office of Aceh on 2 broilers and 1 native chicken.&#13;
Prior to necropsy, preparation of tools and materials for necropsy and sample&#13;
collection were carried out. Necropsy was started with examination of signal,&#13;
anamnesa and carcasses, then necropsy was examined and changes were recorded&#13;
in organs and finally the collection of organs trachea, lungs, duodenum,&#13;
proventriculus, tonsil and brain. Veterinarians determine the diagnosis of&#13;
Newcastle Disease in all birds found based on the presence of hemorrhage /&#13;
hypermi lesions in the tracheal, duodenal, proventricular, tonsil and brain organs.&#13;
Organ fixation for histopathological testing using NBF 10% neutral formalin&#13;
buffer. Veterinary paramedic skills and knowledge about the preparation of the&#13;
tools needed, the implementation of appropriate necropsy procedures and&#13;
collection of samples consisting of tracheal organs, lungs, proventriculus,&#13;
intestines and brain in a 10% NBF solution is very supportive in the diagnosis of&#13;
Newcastle disease in the field.&#13;
&#13;
Keywords : Necropsy, Newcastle disease, Histopathological sample collection. &#13;
&#13;
&#13;
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