Electronic Theses and Dissertation

ABSTRAK
Metabolit sekunder daun pala diisolasi dengan maserasi 3500 g serbuk daun pala
menggunakan pelarut metanol dan diperoleh ekstrak sebanyak 535,7 g (15,3%) dengan
nilai IC
50
188,4 ppm, dilanjutkan partisi dengan pelarut n-heksana, etil asetat dan
kloroform, diperoleh ekstrak masing-masing 1,8488 g (0,35%), 0,501g (0,15%) dan
7,122 g (2,28%) dengan nilai IC
50
96,67 ppm, 0,216 ppm dan 35,62 ppm. Ekstrak nheksana

dan kloroform diisolasi lebih lanjut dengan kromatografi kolom gravitasi
menggunakan eluen masing-masing n-heksana : etil asetat (9:1) dan kloroform : etil
asetat (8:2) dengan hasil diperoleh masing-masing 7 fraksi gabungan (MFHD 1-7) dan 6
fraksi gabungan (MFKD 1-6) dimana seluruh fraksi gabungan aktif sebagai antioksidan.
Fraksi gabungan dengan aktivitas tertinggi yaitu MFHD 2 (IC
50
96,716 ppm), MFHD 4
(IC
50
0,729 ppm), MFKD 4 (IC
50
69,132 ppm) dan MFKD 5 (IC
50
direkromatografi dan dimurnikan dengan KLT preparatif, diperoleh fraksi dengan pola
noda tunggal pada kromatogram KLT yaitu MFHD 2.3 (5,4 mg), MFHD 4.4 (10,7 mg)
dan MFKD 4.4 (5,8 mg) dengan IC
50
masing-masing 42,299 ppm, 44,084 ppm dan
111,91 ppm. Hasil analisis GC-MS fraksi MFHD 2.3 mengandung senyawa eucalyptol
dan sitostenone. Berdasarkan beberapa referensi kedua senyawa tersebut memiliki
aktivitas antioksidan.

Kata Kunci: Daun pala (Myristica fragrans Houtt), Aktivitas antioksidan



80,44 ppm)

ABSTRACT
Isolation of secondary metabolites nutmeg leaf maceration begins with 3500 g nutmeg
leaf powder using methanol and obtained extract as much as 535.7 g (15.3%) with IC
50

value of 188.4 ppm, followed partition with n-hexane, ethyl acetate and chloroform,
each extract was obtained 1.8488 g (0.35%), 0,501g (0.15%) and 7.122 g (2.28%) with
IC
50
value of 96.67 ppm, 0.216 ppm and 35.62 ppm. The extract n-hexane and
chloroform were isolated further by column chromatography gravity using eluent each
n-hexane : ethyl acetate (9 : 1) and chloroform : ethyl acetate (8 : 2) with the results
obtained by each of the 7 fractions combined (MFHD 1-7) and 6 fractions combined
(MFKD 1-6) in which all of the combined fractions are active as antioxidants. The
combined fraction with the highest activity MFHD 2 (IC50 96.716 ppm), MFHD 4
(IC50 0,729 ppm), MFKD 4 (IC50 69.132 ppm) and MFKD 5 (IC50 80.44 ppm) return
column chromatography gravity and purified by preparative TLC, the fraction with a
pattern single spot on TLC chromatograms are MFHD 2.3 (5.4 mg), MFHD 4.4 (10.7
mg) and MFKD 4.4 (5.8 mg) with IC
50
respectively 42.299 ppm, 44.084 ppm and
111.91 ppm. The GC-MS analysis of MFHD 2.3 fraction contains eucalyptol and
sitostenone compounds. Based on some references both of these compounds have
antioxidant activity.
Keywords: Nutmeg leaf (Myristica fragrans Houtt), antioxidant activity

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